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Is blood agar an alternative to sabouraud dextrose agar for the isolation of fungi in patients with mycotic keratitis |
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| Authors: | Ashok Kumar Reddy Upputuri Brahmaiah Nitesh Narayen Ravi Kumar Reddy Rupak Kumar Reddy Meghraj Chitta Srinivas Prasad Rishi Swarup Syed Maaz Mohiuddin Madhukar Reddy Murali K. Aasuri B. S. R. Murthy Milind Bhide Sajid Ahmed |
| Affiliation: | 1. Department of Microbiology, GHR Micro Diagnostics, Dwarakapuri Colony, Punjagutta, Hyderabad, India 2. M.N.R Medical College, Sangareddy, India 3. Maxivision Eye Centre, Hyderabad, India 4. Medivision Eye and Health Care Centre, Hyderabad, India 5. Sarojini Devi Eye Hospital, Hyderabad, India 6. Swarup Eye Centre, Hyderabad, India 13. Neo Retina, Hyderabad, India 7. Eye Care Hyderabad, Hyderabad, India 8. Drishti Eye Centre, Hyderabad, India 9. Sulochana Eye Centre, Hyderabad, India 10. Vasan Eye Care, Hyderabad, India 11. Hyderabad Eye Centre, Hyderabad, India 12. Arvind Eye Hospital, Hyderabad, India |
| Abstract: | To compare the blood agar (BA), sabouraud dextrose agar (SDA) and chocolate agar (CA) for the isolation of fungi in patients with mycotic keratitis. Corneal Scrapings of 229 patients with clinically diagnosed microbial keratitis were inoculated on BA, SDA, CA. The culture media were evaluated for the rate and time taken for the fungal growth. Seventy six of 229 patients had fungal keratitis. Fungus grew on BA in 60/76(78.9 %), on SDA in 76/76 (100 %), on CA in 40/76(52.6 %) patients. The fungi which grew on BA (60/76) also grown on SDA at the same time. The colony morphologies of different fungi were better on SDA than BA/CA. Among the different culture media, SDA is essential for the isolation fungi in patients with mycotic keratitis. |
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