菊淀粉型巴戟天寡糖抗创伤后应激障碍行为学评价

目的 用大鼠和小鼠创伤后应激障碍(PTSD)模型评价菊淀粉型巴戟天寡糖(IOMO)对场景恐惧行为和焦虑行为的影响.方法 建立大鼠单次延长应激(SPS)模型和小鼠不可逃避足底电击(FS)模型,实验分组均为正常对照组、模型组、模型+舍曲林(Ser)组和模型+不同剂量IOMO组.在大鼠SPS模型中,于造模后每天1次ig给予I...

Behavioral evaluation of anti-post-traumatic stress disorder effect of inulin-type oligosaccharides of Morinda officinalis

OBJECTIVE To investigate the effect of inulin-type oligosaccharides of Morinda officinalis(IOMO)on contextual fear and anxiety behavior with post-traumatic stress disorder(PTSD)models of rats and mice.METHODS A single prolonged stress(SPS)model of rats and an inescapable electric foot-shock(FS)model of mice were established.The rats and mice were respectively grouped into normal control group,model group,model+sertraline(Ser)group and model+different doses of IOMO group.In the rat SPS model,IOMO 12.5,25 and 50 mg·kg^-1 or Ser 15 mg·kg^-1 was ig given to the rats,respectively,for sixteen consecutive days.In the FS model of mice,the mice were ig given IOMO 25 and 50 mg·kg^-1 or Ser 15 mg·kg for fifteen consecutive days after foot shock.Contextual freezing measurement was used to evaluate fear behaviors.The elevated plus maze test(EPMT)and open field test(OFT)were used to evaluate the changes in anxiety-like behaviors.RESULTS In the rat SPS model,compared with the normal control group,the freezing time of rats in the model group was significantly increased in the contextual fear test(P<0.01),so was the latency to enter the central area(P<0.05),and the total number of times of entry was significantly reduced(P<0.01)in the OFT.The percentage of time spent in the open arms and times of entry into the open arms in the EPMT were both significantly reduced(P<0.05).Ser 15 mg·kg^-1 and IOMO 50 mg·kg^-1 significantly reversed these changes.In the mouse FS model,the freezing time was significantly increased in the contextual freezing test(on the10th day after foot shock,P<0.01;on the 15th day after foot shock,P<0.05),so was the latency to enter the central area,but there was a significant decrease in the total number of times of entry and the time spent in the central area(P<0.05)in the OFT.Chronic treatment with IOMO 50 mg·kg^-1 could reverse the changes mentioned above.CONCLUSION Chronic treatment with IOMO can significantly reduce contextual fear and anxiety behaviors in SPS model rats and FS model mice,suggesting that IOMO can exert some therapeutic effect on PTSD.