NOTUM基因在骨肉瘤中的表达及临床意义

目的探究NOTUM基因在骨肉瘤中的表达及临床意义。方法选取2011年3月至2014年4月来该院就诊的68例骨肉瘤患者,术中取其癌组织作为试验组,取其正常骨骼肌组织作为对照组。采用实时荧光定量PCR(qRT-PCR)检测NOTUM mRNA表达情况,采用免疫组织化学法检测NOTUM蛋白表达情况,采用Cox回归分析影响骨肉瘤的预后因素。分析NOTUM表达与骨肉瘤病理特征的关系,以及NOTUM表达对骨肉瘤患者生存情况的影响。结果试验组NOTUM mRNA和NOTUM蛋白表达均明显高于对照组,差异均有统计学意义(P<0.05);骨肉瘤患者癌组织中NOTUM蛋白阳性率与患者年龄、性别、肿瘤部位、肿瘤直径和病理类型无关(P>0.05),与Enneking分期和远处转移有关(P<0.05);NOTUM蛋白阴性患者5年生存率明显高于阳性患者,差异有统计学意义(P<0.05);Cox回归分析显示,NOTUM表达、Enneking分期和远处转移均是影响骨肉瘤患者预后的危险因素(P<0.05),其中NOTUM表达和远处转移均是影响骨肉瘤患者预后的独立危险因素。结论骨肉瘤患者癌组织中NOTUM呈高表达,其表达与Enneking分期和远处转移有关,是影响骨肉瘤患者预后的独立危险因素,且与患者预后密切相关,有望成为骨肉瘤的潜在治疗靶点。

Expression and clinical significance of NOTUM gene in osteosarcoma

Objective To investigate the expression of NOTUM gene in osteosarcoma and its clinical significance.Methods Totally 68 patients with osteosarcoma in our hospital from March 2011 to April 2014 were selected,and the cancer tissue was taken as experimental group during operation,and the normal skeletal muscle tissue was taken as the control group.Real-time fluorescence quantitative PCR(qRT-PCR)and immunohistochemistry was used to detected the mRNA level and protein expression level of NOTUM.Cox regression analysis was used to analyze the prognostic factors of osteosarcoma.The relationship between NOTUM expression and pathological features of osteosarcoma and the influence of NOTUM expression on the survival of osteosarcoma patients were analyzed.Results The mRNA and protein expressions level of NOTUM in the experimental group were significantly higher than those in the control group(P<0.05).The positive rate of NOTUM protein in osteosarcoma tissue was not related to age,sex,location,diameter and pathological type(P>0.05),but was related to Enneking stage and distant metastasis(P<0.05).The 5-year survival rate of NOTUM protein-negative patients was significantly higher than that of NOTUM protein-positive patients(P<0.05).Cox regression analysis showed that the expression of NOTUM,Enneking stage and distant metastasis were risk factors for the prognosis of osteosarcoma patients(P<0.05),and the expression of NOTUM and distant metastasis were independent risk factors for the prognosis of osteosarcoma patients.Conclusion NOTUM is high expression in osteosarcoma tissue,which is related to Enneking stage and distant metastasis.Meanwhile,it is an independent risk factor affecting the prognosis of osteosarcoma patients and is closely related to the prognosis of patients,so it is expected to become a potential therapeutic target for osteosarcoma.