两种多重核酸血液筛查系统在血液筛查应用中的比较

目的通过对两种多重核酸血液筛查系统临床数据分析比较,为血站系统的使用提供参考。方法汇总2018年1-10月哈尔滨市血液中心采用北京万泰和罗氏多重核酸血液筛查系统进行检测的数据及部分阳性标本跟踪数据,统计分析这两个检测系统的性能差异及原因。结果万泰和罗氏核酸检测系统分别检测86 475份和36 641份标本,万泰系统的乙型肝炎病毒(HBV)假阳性率低于罗氏,罗氏系统的丙型肝炎病毒(HCV)和人类免疫缺陷病毒(HIV)假阳性率低于万泰;两个系统的HBV、HCV和HIV的阳性率基本一致。罗氏系统的无效率高于万泰系统。结论两个多重核酸血液筛查系统各有优劣,具有一定互补性,但均能从ELISA阴性标本中检测出一定数量的核酸阳性标本,检出率基本一致,均能提高输血安全性。

Comparison between two kinds of multiple nucleic acid blood screening systems in blood screening application

Objective To compare and analyze the clinical data of two kinds of multiple nucleic acid screening system to provide reference for the use by the blood stations system.Methods The detection data of the Beijing Wantai and Roche multiple nucleic acid blood screening reagents and partial positive sample tracking data from January to October 2018 were summarized for statistically analyzing the performance difference and causes of these two detection systems.Results The Wantai and Roche nucleic acid detection system detected86 475 specimens and 36 641 specimens respectively.The hepatitis B virus(HBV)false positive ratio of the Wantai system was lower than that of the Roche system,while the false positive ratios of hepatitis C virus(HCV)and human immunodeficiency virus(HIV)in The Roche system were lower than those in the Wantai system.The positive rates of HBV,HCV and HIV in these two systems were basically consistent.The inefficiency rate of the Roche system was higher than that of the Wantai system.Conclusion The two kinds of multiple systems Wantai and Roche have their own advantages and disadvantages,possess some extent complementarity,but which can detect a certain number of nucleic acid positive samples from the negative samples detected by ELISA,the detection rates are basically consistent,the both system can increase the blood transfusion safety.