烧伤科患者分离金黄色葡萄球菌耐药性和耐药基因分析

目的了解该院烧伤科患者创面分离的金黄色葡萄球菌的耐药性和耐药基因分布,为金黄色葡萄球菌在烧伤感染方面的研究提供实验室依据。方法收集2018年9月至2019年5月该院烧伤科分离的135株金黄色葡萄球菌,采用VITEK-2 Compact系统和纸片扩散法进行菌种鉴定和药敏试验,同时检测其耐药基因。结果135株金黄色葡萄球菌对利奈唑胺、万古霉素、替考拉宁、奎奴普汀/达福普利和替加环素的敏感率均为100.0%。对青霉素G的耐药率为97.0%,对庆大霉素、红霉素、克林霉素和四环素耐药率均超过60.0%;ermA、ermB、ermC的阳性率分别是51.9%、28.1%和62.2%;mecA、TEM、ant6、aac6′/aph2′基因的阳性率为54.1%、32.6%、17.0%和62.2%。结论烧伤科患者分离的金黄色葡萄球菌对多数抗菌药物都有比较高的耐药性,对利奈唑胺、万古霉素、替考拉宁、奎奴普汀/达福普利和替加环素高度敏感,耐药基因以mecA、ermA、ermC和aac6′/aph2′为主。

Drug resistance and resistance gene analysis of Staphylococcus aureus isolated from patients in the department of burns

Objective To understand the distribution of drug resistance and resistance genes of Staphylococcus aureus isolated from wounds of patients in the department of burns of our hospital,so as to provide laboratory basis for the study of Staphylococcus aureus in burn infection.Methods Totally 135 strains Staphylococcus aureus isolated from patients in the Department of Burns of our hospital from September 2018 to May 2019 were collected.The strain identification and drug susceptibility test were conducted by using the VITEK-2 Compact system and the disk diffusion method.The drug-resistant genes were expressed by PCR amplification,and the drug resistance and drug resistance genes were statistically analyzed.Results The sensitivity of 135 strains Staphylococcus aureus to linezolid,vancomycin,ticolanin,quinuptin/dafuopril and tegacycline were 100.0%.The drug resistance rate to penicillin G was 97.0%,and the drug resistance rate to gentamicin,clindamycin,erythromycin and tetracycline were more than 60.0%.The positive rates of ermA,ermB and ermC were 51.9%,28.1%and 62.2%,respectively.The positive rates of mecA,TEM,ant6,aac6′/aph2′genes were 54.1%,32.6%,17.0%and 62.2%,respectively.Conclusion Staphylococcus aureus isolated from patients in the department of burns has a high drug resistance rate to most antimicrobial agents,and it is highly sensitive to linezolid,vancomycin,ticolanin,quinuptin/dafuopril and tegacycline.Drug resistance genes are mainly mecA,ermA,ermC and aac6′/aph2′.