Isolation and characterization of 8 polymorphic microsatellite markers from the Greater Short-horned Lizard (Phrynosoma hernandesi)

The Greater Short-horned Lizard (Phrynosoma hernandesi) is a small reptile endemic to western North America that is classified as endangered in Canada. Here, we describe the development of 8 microsatellite markers for P. hernandesi. Observed allelic diversity varied from 2 to 11 while observed heterozygosity and expected heterozygosity ranged from 0.370–0.852 to 0.486–0.856, respectively. These markers will facilitate studies that address conservation of the species, such as levels of genetic diversity and population structure.