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Development of anonymous nuclear markers from Illumina paired-end data for Seychelles caecilian amphibians (Gymnophiona: Indotyphlidae) |
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| Authors: | Claire J. Lewis Simon T. Maddock Julia J. Day Ronald A. Nussbaum Charles Morel Mark Wilkinson Peter G. Foster David J. Gower |
| Affiliation: | 1. Department of Life Sciences, The Natural History Museum, London, SW7 5BD, UK 2. Department of Genetics, Evolution and Environment, University College London, London, WC1E 6BT, UK 3. Museum of Zoology, University of Michigan, Ann Arbor, MI, 48109, USA 4. Natural History Museum, Victoria, Mahé, Seychelles |
| Abstract: | Anonymous nuclear markers were developed for Seychelles caecilian amphibians. Using a previously published bioinformatics pipeline (developed for Roche 454 data), 36 candidate anonymous nuclear loci (ANL) of at least 180 bp length were identified from Illumina MiSeq next generation sequencing data for five Seychelles species. We designed primer pairs for the 36 candidate ANL and tested these by PCR and Sanger sequencing. Seven ANL amplified and sequenced well for at least five of the six nominal Seychelles caecilian species (in three genera), and represent potentially useful markers for systematics and conservation. |
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