血管内皮生长因子C及其受体表达与乳腺癌淋巴结转移的相关性研究

目的:本研究旨在检测乳腺癌组织中VEGF-C/VEGFR-3、LVD的表达情况,探讨其表达水平与临床病理特征、复发转移的相互关系,进一步研究肿瘤转移的机制,为抗淋巴管形成、进而为抗乳腺癌淋巴转移的治疗提供理论依据。方法:应用免疫组织化学(SP)法检测83例乳腺癌手术标本的原发灶的VEGF-C及其受体VEGFR-3的表达及LVD。结果:83例乳腺癌组织VEGF-C阳性表达者36例(43.3%),淋巴结转移组的VEGF-C阳性表达率为59.4%(24/44),显著高于无淋巴结转移组30.8%(24/44),(P<0.05)。VEGF-C表达与年龄、临床分期和激素受体ER、PR、CerbB-2表达无关;LVD与年龄、临床分期和ER、PR、CerbB-2表达水平无关;在VEGF-C表达阳性组、阴性组中LVD统计学上差异显著(P<0.05)。结论:乳腺癌组织VEGF-C表达可能促进肿瘤内淋巴管的生成;乳腺癌的淋巴结转移和VEGF-C的高表达密切相关。

Relationship of the Expressions of Vascular Endothelial Growth Factor-C and its Receptor with the Metastasis of Lymph Node in Mammary Cancer

Objective:To determine the expressions of vascular endothelial growth factor-C (VEGF-C)/its receptor vas- cular endothelial growth factor-3(VEGF-3) and LVD,and investigate the interactions of the expressions with the clinicopatho- logical characteristics,recurrence and metastasis,and further study the mechanism of tumor metastasis,so as to provide theo- retical basis for treating against formation of lymphatic vessel,and against the lymphatic metastasis in mammary cancer.Methods:The expressions of VEGF-C and its receptor VEGF-3 in the primary focus and LVD were detected using im- munohistochemical method (SP method) in the operated samples of 83 cases of mammary cancer.Results:In the 83 tissues of mammary cancer,VEGF-C positively expressed in 36 cases (43.3%).The positive rate of VEGF-C expression in the lymphatic metastasis group (59.4%,24/44) was significantly higher than that in the without lymphatic metastasis group (30.8%,24/ 44) (P<0.05).VEGF-C expression and LVD had no association with age,clinical stage,hormone receptor ER,PR and CerbB-2 expressions.LVD was significantly different between VEGF-C positive expression group and VEGF-C negative expres- sion group (P<0.05).Conclusion:VEGF-C expression in mammary cancer tissue may accelerate the formation of lymphatic vessel in tumor.The metastasis of lymph node in mammary cancer has close correlation with the high expression of VEGF-C.