miR-154对前列腺癌细胞功能影响的实验研究

目的 研究miR-154在对前列腺癌组织中的表达及其对前列腺癌细胞生物学功能的影响.方法 采用RT-PCR方法检测前列腺癌和癌旁正常组织样本中miR-154的表达.运用细胞增殖实验(CCK-8法)、克隆形成实验及细胞周期分析评估上调miR-154对前列腺细胞系DU145和PC-3的作用.结果 与癌旁正常前列腺组织相比,miR-154在前列腺癌组织中的表达水平显著下调.体外实验中,miR-154过度表达能显著抑制癌细胞的生长,细胞克隆形成数明显降低.流式细胞术检测发现癌细胞的生长受到抑制,在G0/G1期比率明显升高,而上调miR-154可以使前列腺癌细胞阻滞在G1期,而抑制细胞的增殖.结论 miR-154可以影响前列腺癌细胞的增殖功能,有望成为前列腺癌治疗的新靶点.

Inhibitive effect of miR-154 on the proliferation of prostate cancer cells

Objective To investigate the expression of miR-154 in prostate cancer and its effect on prostate cancer （PCa） cell lines. MethodsmiR-154 expressions in primary PCa samples and non malignant sample tissues were measured by qRT PCR. Effects of miR-154 on cell proliferation in vitro were evaluated by CCK-8 assay, colony formation assay, and Flow cytometry, respectively. Results miR-154 expression was significantly decreased in primary PCa samples compared to non malignant samples. Restoration of miR-154 decreased the potential of cell growth and proliferation in prostate cancer cell lines in vitro. Conclusions These findings suggest that miR-154 plays a prominent role in prostate cancer proliferation and may provide a new approach for the treatment of prostate cancer.