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Longitudinal study of t-cell somatic mutations conferring glycosylphosphatidylinositol-anchor deficiency in gulf war I veterans exposed to depleted uranium |
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| Authors: | Richard J. Albertini Janice A. Nicklas Pamela M. Vacek Elizabeth W. Carter Melissa McDiarmid |
| Affiliation: | 1. Department of Pathology, University of Vermont College of Medicine, Burlington, Vermont;2. Department of Pediatrics, University of Vermont College of Medicine, Burlington, Vermont;3. Medical Biostatistics Unit, University of Vermont College of Medicine, Burlington, Vermont;4. Jeffords Institute for Quality, University of Vermont Medical Center, Burlington, Vermont;5. Occupational Health Program, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland U.S. Department of Veterans Affairs, Washington, DC |
| Abstract: | Fifty Veterans of the first Gulf War in 1991 exposed to depleted uranium (DU) were studied for glycosylphosphatidylinositol-anchor (GPIa) deficient T-cell mutants on three occasions during the years 2009, 2011, and 2013. GPIa deficiency was determined in two ways: cloning assays employing aerolysin selection and cytometry using the FLAER reagent for positive staining of GPIa cell surface proteins. Subsequent molecular analyses of deficient isolates recovered from cloning assays (Nicklas JA et al. [2019]: Environ Mol Mutagen) revealed apparent incomplete selection in some cloning assays, necessitating correction of original data to afford a more realistic estimate of GPIa deficient mutant frequency (MF) values. GPIa deficient variant frequencies (VFs) determined by cytometry were determined in the years 2011 and 2013. A positive but nonsignificant association was observed between MF and VF values determined on the same blood samples during 2013. Exposure to DU had no effect on either GPIa deficient MF or VFs. Environ. Mol. Mutagen. 60:494–504, 2019. © 2019 Wiley Periodicals, Inc. |
| Keywords: | PIGA GPI anchor Gulf War veterans depleted uranium in vivo somatic mutations |