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| 单克隆与多克隆双抗体夹心法测定血清可溶性IL-2受体 | |
| 引用本文： | 富宁,王莉,杨贵贞. 单克隆与多克隆双抗体夹心法测定血清可溶性IL-2受体[J]. 中国免疫学杂志, 1991, 0(5) |
| 作者姓名： | 富宁 王莉 杨贵贞 |
| 作者单位： | 白求恩医科大学免疫学教研室，白求恩医科大学免疫学教研室，白求恩医科大学免疫学教研室 长春 130021，长春 130021，长春 130021 |
| 摘 要： | 建立了灵敏的酶标双抗体夹心法测定血清可溶性IL-2受体(sIL-2R)。主要步骤为以单抗包被,加入待检抗原,再加多克隆兔抗IL-2 R血清作用,最后加酶标羊抗兔示踪并放大其反应。本法简便、重复性好、灵敏度达100u/ml.可适用于临床与基础研究。 |
| 关 键 词： | 双抗体夹心 ELISA 溶性IL-2受体 |
Measurement of soluble IL-2 receptor by sandwich ELISA with monoclonal and polyclonal antibodies |
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| FU Ning,et al.. Measurement of soluble IL-2 receptor by sandwich ELISA with monoclonal and polyclonal antibodies[J]. Chinese Journal of Immunology, 1991, 0(5) | |
| Authors: | FU Ning et al. |
| Affiliation: | FU Ning,et al. Department of Immunology,Norman Bethune University of Medical Sciences,Changchun 130021 |
| Abstract: | In this work, a simple and sensitive assay for soluble IL-2 receptor (sIL-2R) was established. The main procedures are as follows: the microplate was coated with anti-Tac monoclonal antibody (McAb); after sIL-2R in samples bound with the McAb, the rabbit anti-IL-2R serum was added;and HRP-goat anti-rabbit IgG was used to display and amplify this binding. The sensitivity of this method is about 100u/ml, which is near that in "Cellfree IL-2 Receptor Test Kit". |
| Keywords: | soluble IL-2 receptor sandwich ELISA |
| 本文献已被 CNKI 等数据库收录！ | |