棕树心化学成分研究

目的：研究棕树心的化学成分。方法采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱及重结晶等方法进行分离纯化，通过波谱数据分析和理化性质鉴别进行结构鉴定。结果从棕树心95％乙醇提取物中分离鉴定了10个化合物，分别鉴定为：1，3-二羟基蒽醌（1），大叶茜草素（2），4-羟基-2，6-二甲氧基-苯甲酸（3），5-羟基-2-羟甲基-γ-吡喃酮（4），3，5-二羟基-2-甲基-γ-吡喃酮（5），3，5-二羟基-γ-吡喃酮（6），胡萝卜苷（7），对羟基苯甲酸（8），儿茶酚（9），β-谷甾醇（10）。结论1～7为首次从该植物中分离得到，1～6为首次从棕榈属植物中分离得到。抗肿瘤体外活性筛选结果显示，化合物2对人骨肉瘤细胞株U20 S、人神经母细胞瘤细胞株SY5 Y均有一定的生长抑制作用，其IC50值分别为18．10、26．59μg／ml；化合物5对人乳腺癌细胞株MCF-7的生长有一定的抑制作用，其IC50值为37．31μg／ml。

Chemical constituents from the heartwood of Trachycarpus fortunei (Hook.) H. Wendl

Objective To study the chemical constituents of the heartwood of Trachycarpus fortunei.Methods Compounds were isolated and purified by silica gel column , Sephadex LH-20 chromatography and recrystallization .Their structures were elucidated by physicochemical properties and spectral data .Results Ten compounds were isolated from the 95%ethanol extract of the heartwood of T.fortunei and identified as 1,3-dihydroxyanthraquinone (1),mollugin(2), 4-hydroxy-2, 6-dimethoxy-benzoic acid ( 3 ), 5-hydroxy-2-hydroxymethyl-γ-pyranone ( 4 ), 3, 5-dihydroxy-2-methyl-γ-pyranone(5),3,5-dihydroxy-γ-pyranone (6),daucosterol (7),4-hydroxybenzoic acid (8),pyrocatechol (9),β-sitosterol (10).Conclusion Compounds 1-7 have been obtained from this plant for the first time ,and 1-6 from the genus for the first time.The screening results of antitumor activity in vitro show that the half inhibitory concentration IC 50 of compound 2 for human osteosarcoma cells U20S and human neuroblastoma cells SY 5Y is 18.10 and 26.59 μg/ml respectively. Compound 5 can inhibit the growth of human breast cancer cells MCF-7 with a half inhibitory concentration IC 50 of 37.31μg/ml.