APCmin/+小鼠缺失维生素D受体对肠道肿瘤生长的影响 |
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引用本文: | 康红,郑维,喻宏.APCmin/+小鼠缺失维生素D受体对肠道肿瘤生长的影响[J].中国医师杂志,2009,11(7):1174-1177. |
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作者姓名: | 康红 郑维 喻宏 |
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作者单位: | 湖南省人民医院急诊科,湖南,长沙,410005;湖南省人民医院普外科,湖南,长沙,410005;湖南省人民医院病理科,湖南,长沙,410005; |
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基金项目: | 湖南省教育厅科研基金资助项目湖南省卫生厅科研基金资助项目湖南省人民医院仁术重点科研基金项目 |
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摘 要: | 目的 探讨维生素D受体(VDR)失活对APCmin/+小鼠肠道肿瘤生长的影响及机制.方法 通过构建APCmin/+VDR-/-小鼠模型(n=8),与APCmin/+小鼠比较(n=8),4月龄时观察肠道肿瘤大小及数目情况,肿瘤作HE染色以判断病理类型,免疫组化检测肿瘤相关基因BCL-2、vimentin-1、Stat-1和MSH-2蛋白的表达.结果 4月龄时2组鼠比较,APCmin/+VDR-/-小鼠>3 mm肿瘤明显增多(P<0.01).HE染色显示肠道肿瘤为管状腺瘤.APCmin/+肿瘤的Stat-1表达较强,而MSH-2和vi-mentin-1表达在APCmin/+VDR-/-肿瘤中均更强.结论 维生素D受体缺失促使APCmin/+小鼠肠道肿瘤的发展.
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关 键 词: | 肠肿瘤/遗传学 受体 骨化三醇/生物合成 基因 bcl-2/生物合成 中间丝蛋白质类/生物合成 原癌基因蛋白质类/生物合成 |
Effects of inactivation of vitamin D receptor in intestinal tumor growth of APCmin/+ mice |
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Abstract: | Objective To explore effects and mechanism of inactivation of vitamin D receptor (VDR) in intestinal tumor growth of APCmin/+ mice. Methods To generate APCmin/+VDR -/- mice through breeding, the number and size of small intestinal and colonic tumors were assessed and compared between APCmin/+ and APCmin/+ VDR -/- mice. The intestinal tumors were diagnosed with HE stain. The expressions of BCL-2,vimentin-1,Stat-1 and MSH-2 proteins of tumors were determined by immanohistocbemistry and compared be-tween APCmin/+ and APCmin/+VDR -/- mice. Results A comparison between APCmin/+ and APCmin/+ VDR -/- mice revealed that the number of the tumors, which were larger than 3mm, was significantly increased in APCmin/+ VDR -/- mice at 4 months of age (P< 0.01). HE staining indicated fistulous adenomas from small intestine and colon of two groups, Immunostaining showed Stat - 1 level in APC-min/+ tumors were increased and MSH-2 and vimentin-1 levels in APCmin/+ VDR-/- mice were increased, compared to APCmin/+tumors. Conclusion These observations suggested that inactivation of VDR promoted the intestinal tumor growth of APCmin/+ mice. |
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Keywords: | Intestinal neoplasms/GEReceptorscalcitrioL/BIGenesbcl-2/BIIntermediate filament proteins/BIProto-onco-gene proteins/Bl |
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