人子宫内膜异位症异位及在位内膜间质细胞分离与培养

目的：建立子宫内膜异位症（EMs）异位、在位内膜间质细胞的体外细胞模型，实时观察间质细胞形态变化。方法胶原酶消化异位、在位内膜组织，二次筛网过滤结合低速离心法分离纯化间质细胞，应用免疫细胞化学染色进行细胞鉴定，并进行传代培养，观察细胞生长状况及细胞形态差异。结果培养成功率91．3％，间质细胞纯度达95％；异位间质细胞9代内、在位及对照组间质细胞11代内，细胞形态及活性并无明显改变。结论子宫内膜间质细胞可为子宫内膜异位症研究提供较好的细胞模型。

Isolation and culture of human ectopic and eutopic endometrial stromal cells

Objective To establish the in vitro cell model of ectopic and eutopic endometrial stromal cells in endometriois (EMs) for conducting the real time observation on the morphological changes of stromal cells .Methods The ectopic and eutopic endometrial stromal cells were digested by collagenase ,isolated and purified by the quadratic sieve filtration combined with the low speed centrifugation .The cells were identified by the immunohistochemistry staining .The cells passage culture was performed .The cells growth condition and differences in cell morphology were observed .Results The culture success rate of endometrial stromal cells was 91 .3% .The stromal cells purity reached 95% .The cell morphology and activity had no obvious changes for the ectopic endometrial stromal cells within 9 generations ,and the eutopic endometrial stromal cells and the control stromal cells within 11 gen‐erations .Conclusion The endometrial stromal cells can provide better cell model for the research of EMs .