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| HPLC法测定N393的含量 | |
| 引用本文： | 张波,窦嫒嫒,王洪权,张东娜. HPLC法测定N393的含量[J]. 解放军药学学报, 2009, 25(4): 353-354 |
| 作者姓名： | 张波 窦嫒嫒 王洪权 张东娜 |
| 作者单位： | 军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京,100071;北京军区联勤部药品仪器检验所,北京,100071 |
| 摘 要： | 目的采用HPLC法测定N393的含量。方法选用迪马-C18（250mm×4.6mm,5μm）色谱柱,以乙腈-0.01mol.L-1磷酸二氢钾（KH2PO4）溶液（65∶35）为流动相,检测波长：220nm。结果N393在6～280μg·mL-1（r=0.9997）范围内线性关系良好,方法平均回收率为99.72%,RSD为0.67%（n=9）。结论本法可用于测定N393的含量。 |
| 关 键 词： | N393 HPLC 含量测定 |
Determination of the Content of N393 by HPLC |
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| ZHANG Bo,DOU Yuan-Yuan,WANG Hong-Quan,ZHANG Dong-Na. Determination of the Content of N393 by HPLC[J]. Pharmaceutical Journal of Chinese People's Liberation Army, 2009, 25(4): 353-354 | |
| Authors: | ZHANG Bo DOU Yuan-Yuan WANG Hong-Quan ZHANG Dong-Na |
| Affiliation: | (Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, State Key Laboratory of Pathogens and Biosecurity Beijing 100071 China;Institute for Drug and Instrument Control of Beijing Military Command, Beijing 100071 China) |
| Abstract: | Aim To establish an HPLC method for determination of the content of N393.Methods The assay was conducted on a DIMA-C18（250mm×4.6mm,5μm）column with the methyl cyanide-0.01mol·L-1 potassium dihydrogen phosphate（KH2PO4）solution（65∶35）as the mobile phase.The detection wavelength was 220nm.Results The calibration curve was linear over the range 6～280μg·ml-1（r=0.999 7）for N393.The average recovery was 99.72% with RSD=0.67%（n=9）.Conclusion The HPLC method can be used for determination of N393. |
| Keywords: | N393 HPLC |
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