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| 氨甲蝶呤辐射增敏作用与RAD51的相关性研究 | |
| 引用本文： | 杜利清,白剑强,刘强,王彦,赵鹏,陈凤华,王宏,樊飞跃. 氨甲蝶呤辐射增敏作用与RAD51的相关性研究[J]. 中华放射医学与防护杂志, 2012, 32(1): 44-46 |
| 作者姓名： | 杜利清 白剑强 刘强 王彦 赵鹏 陈凤华 王宏 樊飞跃 |
| 作者单位： | 1. 中国医学科学院放射医学研究所分子核医学重点实验室, 天津,300192 2. 天津骨科医院脊柱外科 3. 华北煤炭医学院 |
| 摘 要： | 目的 探讨同源重组修复蛋白RAD51与氨甲蝶呤辐射增敏作用的相关性.方法 分别采用Western blot和RT-PCR法,观察γ射线、氨甲蝶呤及二者联合应用对人骨肉瘤细胞RAD51表达的影响,克隆形成实验观察氨甲蝶呤对RAD51高表达前后骨肉瘤细胞辐射增敏作用的影响.结果 氨甲蝶呤在蛋白质和RNA水平抑制RAD51的表达,氨甲蝶呤和射线联合应用可明显降低RAD51的表达水平；HOS细胞和RAD51高表达的HOS-RAD51细胞加药前后的辐射增敏比分别为1.51和0.99,表明氨甲蝶呤对HOS细胞具有较好的放射增敏性.结论 RAD51可能参与了氨甲蝶呤的辐射增敏作用. |
| 关 键 词： | RAD51 氨甲蝶呤 辐射增敏 |
| 收稿时间： | 2011-04-12 |
Correlation of RAD51 and radiosensitization of methotrexate |
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| DU Li-qing,BAI Jian-qiang,LIU Qiang,WANG Yan,ZHAO Peng,CHEN Feng-hu,WANG Hong and FAN Fei-yue. Correlation of RAD51 and radiosensitization of methotrexate[J]. Chinese Journal of Radiological Medicine and Protection, 2012, 32(1): 44-46 | |
| Authors: | DU Li-qing BAI Jian-qiang LIU Qiang WANG Yan ZHAO Peng CHEN Feng-hu WANG Hong FAN Fei-yue |
| Affiliation: | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China;Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China;Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China;Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China;Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China;Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China |
| Abstract: | |
| Keywords: | RAD51 Methotrexate Radiosensitization |
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