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人视网膜神经胶质细胞原代培养
引用本文:罗贤民,姜德咏,聂爱光. 人视网膜神经胶质细胞原代培养[J]. 中华眼底病杂志, 1995, 11(4): 247-249
作者姓名:罗贤民  姜德咏  聂爱光
作者单位:湖南医科大学第二附属医院眼科
摘    要:
采用组织消化培养法培养山人视网膜神经胶质(retinal glia,RG)细胞,用免疫组织化学技术对细胞进行鉴定,显示培养细胞呈神经胶质纤维酸性蛋白(glial fibrillary acid protein,GFAP),S-100染色阳性。结合透射及扫描电镜观察,证实为人视网膜神经胶质细胞。(中华眼底病杂志,1995,11:247-249)

关 键 词:神经胶质 视网膜 细胞培养 视网膜疾病
收稿时间:1994-12-19
修稿时间:1995-06-12

PRIMARY CELL CULTURE OF HUMAN RETINAL GLIAL CELLS IN VITRO
Luo Xianmin,Jiang Deyong,Nie Aiguang. PRIMARY CELL CULTURE OF HUMAN RETINAL GLIAL CELLS IN VITRO[J]. Chinese Journal of Ocular Fundus Diseases, 1995, 11(4): 247-249
Authors:Luo Xianmin  Jiang Deyong  Nie Aiguang
Affiliation:Dept of Ophthalmology,Second Affiliated Hospital,Hunan Medical University,Changsha 410011
Abstract:
In this experiment,the human relinal glial cells were cultured with tissue digeation procfdures. The cultured cells showed positive reaction to glial fibrillary acid protein(GFAP)and S-100 protein with an immunohistochemical assay, Under the observation of transmission and screening eleclron microscope,the cultured cells were proved to be the retinal glia cells.
Keywords:Neuroglia Retina Cells   cultured  
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