| 桔梗皂苷D对照品的制备 |
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| 引用本文： | 李伟,张晶,李慧萍,王梓,郑毅男. 桔梗皂苷D对照品的制备[J]. 中药新药与临床药理, 2007, 18(1): 55-56 |
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| 作者姓名： | 李伟 张晶 李慧萍 王梓 郑毅男 |
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| 作者单位： | 吉林农业大学中药材学院,长春,130118 |
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| 摘 要： | 目的建立桔梗皂苷D对照品的制备工艺。方法桔梗生药用70%甲醇提取,浓缩后经大孔树脂D101柱色谱,40%乙醇洗脱组分用硅胶柱色谱分离,以氯仿-甲醇系统梯度洗脱,收集含桔梗皂苷D的组分,最后经高效液相色谱制备纯化。结果HPLC检测分析,采用面积归一法计算,桔梗皂苷D纯度为98.8%。结论该工艺简单,收率高,可用于桔梗皂苷D标准品的制备。
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| 关 键 词： | 桔梗皂苷D 分离与制备 |
| 文章编号： | 1003-9783（2007）01-0055-02 |
| 收稿时间： | 2006-09-05 |
| 修稿时间： | 2006-09-05 |
Preparation of Platycodin-D for Reference Substance |
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| LI Wei,ZHANG Jing,Li Huiping,WANG Zi,ZHENG Yinan. Preparation of Platycodin-D for Reference Substance[J]. Traditional Chinese Drug Research & Clinical Pharmacology, 2007, 18(1): 55-56 |
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| Authors: | LI Wei ZHANG Jing Li Huiping WANG Zi ZHENG Yinan |
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| Affiliation: | College of Chinese Material Medicine, Jilin Agricultural University, Changchun 130118 |
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| Abstract: | Objective To establish the preparation techniques for preparing the reference substance of platycodin-D.Methods Raw material of platycodi radix was extracted with 70 %MeOH.The concentrated extract passed through D101 macroporous resin column,then the 40 %alcohol eluate was separated by silica gel column chromatography with chloroform-methanol gradient elution.The fractions contained platycodin-D were collected and purified by HPLC.Results HPLC analysis and normalization of peak areas showed the purity of the platycodin-D was 98.8 %.Conclusions The preparation techniques are simple and high yield,can be used for preparing the reference substance of platycodin-D. |
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| Keywords: | HPLC |
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