舒胸片中人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1含量测定

目的：建立舒胸片中人参皂苷Rg1、人参皂苷Rb，和三七皂苷R1含量的HPLCI]定方法。方法：采用DiamosilC18柱（4．6X250mm，5gm），流动相乙腈一水，梯度洗脱，柱温30℃，流速lmL／min，203nm波长下检测。结果：人参皂苷Rg1、人参皂苷Rb1、三七皂苷R，分别在0．442～11．050gg（r=0．9999）、0．344～8．600μg（P=0．9999）、0．208～5．200gg（r=0．9995）范围内线性关系良好，平均回收率分别为98．93％（RSD为1．7％1、98．88％（RSD为1．4％）、98．98％（RSD为1．4％）。结论：以HPLC法检测舒胸片中人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1，方法简便、准确、灵敏度高、重复性好。

Qualification and Quantification of Ginenoside Rg1, Rb1 and Notoginsenoside R1 in ShexiongTablet by HPLC

Objective： To develop a HPLC method for the qualification and quantification of ginsenoside Rg1, Rb, and notoginsenoside R, in Shuxiong Tablet. Methods： The column was Diamosil C18 （4.6 x 250mm, 5gm）. The gradient elution mixture consisted of acetonitrile and water. The flow rate was lmL/min, and the column temperature and detection wavelength were 30℃ and 203 nm, respectively. Results： Linear ranges were 0.442 11.050 μg （1=0.9999） for ginsenoside Rg,, 0.344- 8.600 μg （r=0.9999） for ginsenoside Rb1, 0.208- 5.200 gg （r=0.9995） for notoginsenoside R,. The average recovery was 98.93% with RSD 1.7% for ginsenoside Rg1, 98.88% with RSD 1.4% for ginsenoside Rbl, 98.98% with RSD 1.4% for notoginsenoside R1. Conclusion： The established HPLC method is convenient, accurate, sensitive and repeatable.