Silica Nanoparticles Encapsulated Cichorium Pumilum as a Promising Photosensitizer for Osteosarcoma Photodynamic Therapy: In-vitro study
Institution:
1. Department of Physics, Bio-Medical Physics Laboratory, Jordan University of Science and Technology, Jordan;2. Medical Imaging and radiography Department, Aqaba University of Technology, Aqaba, Jordan;3. School of Physics, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia;4. NanoBiotechnology Research and Innovation (NanoBRI), Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 USM, Penang, Malaysia;1. Faculty of Medicine, Department of Ophthalmology, Erciyes University, Kayseri, Turkey;2. Faculty of Medicine, Department of Physical Medicine and Rehabilitation, Marmara University, ?stanbul, Turkey;3. Ulucanlar Eye Training and Research Hospital, Ankara, Turkey;1. Department of Ophthalmology, Alanya Alaaddin Keykubat University Faculty of Medicine, Antalya, Turkey;2. Department of Ophthalmology, Health Sciences University Antalya Training and Research Hospital, Antalya, Turkey;3. Department of Rheumatology, Health Sciences University Antalya Training and Research Hospital, Antalya, Turkey;4. Department of Internal Medicine, Health Sciences University Antalya Training and Research Hospital, Antalya, Turkey;1. Department of Gastroenterology and Hepatology, Osaka Rosai Hospital, Sakai, Osaka, Japan;2. Department of Pathology, Osaka Rosai Hospital, Sakai, Osaka, Japan;1. Eye Unit, Department of Medicine, Surgery and Dentistry, \"Scuola Medica Salernitana\", University of Salerno, Baronissi, Italy
Abstract:
Background/AimsSilica nanoparticles (SiNPs) have been promising vehicles for drug delivery. Cichorium Pumilum (CP), a natural photosensitizer (PS), has been reported to have many useful effects in cancer treatment. However, the poor water solubility and its low bioavailability have confined its use as a suitable photosensitizer for photodynamic therapy. Therefore, a subtle approach is required to overcome these drawbacks.Materials and MethodsWe have synthesized a silica nanoparticles loaded with Cichorium Pumilum. The nanoparticles structural morphologies have been charectrized by Transmission Electron Microscopy (TEM). The cytotoxicity for different concentrations of naked and encapsulated CP was evaluated. Moreover, the optimal concentration of naked and encapsulated CP with exposure time to a light (Maximum intensity at 350nm ～0.27mW/cm2) required to eliminate the used cells (Osteosarcoma cells) were also measured.ResultsThe results showed that encapsulated CP in SiNPs exhibited relatively higher efficacy than the naked CP by + 157.14 % of exposure time efficacy and + 49.45% of concentration efficacy, and encapsulated CP was also confirmed to be effective in eradicating osteosarcoma cells.ConclusionThe engineered silica nanoparticles loaded with CP enhanced the photodynamic therapy by increasing the CP bioavailability.
