MicroRNAs as serum biomarker for Senecio brasiliensis poisoning in cattle |
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| Institution: | 1. Department of Agriculture, Biodiversity and Forest, Postgraduate Program in Conventional and Integrative Veterinary Medicine, Curitibanos, SC 89520-000, Brazil;2. Postgraduate Program in Pharmacy, Federal University of Santa Catarina, Florianópolis, SC 88040-900, Brazil;3. Department of Pharmaceutical Sciences, Federal University of Santa Catarina, Florianópolis, SC 88040-900, Brazil;4. VERTÁ, Laboratory of Veterinary Diagnostic, Institute of Veterinary Research and Diagnostic, Curitibanos, SC 89520-000, Brazil;1. College of Architecture and Civil Engineering, Xi’an University of Science and Technology, Xi’an 710054, Shaanxi, China;2. Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Engineering Technology Research Center for Wastewater Treatment and Reuse, Key Laboratory of Environment Engineering, Shaanxi, Province, Xi’an University of Architecture and Technology, Xi’an 710055, Shaanxi, China;3. College of Geography and Environment, Baoji University of arts and sciences, Baoji, Shaanxi 721013, China;4. Shandong Dongyuan New Material Technology Co., Ltd, Dongying 257300, Shandong, China;1. Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, RJ 21.941-902, Brazil;2. Department of Biological Science, University of Calgary, Calgary, AB T2N 1N4, Canada;1. Facultad de Ciencias, Departamento de Farmacia, Universidad Nacional de Colombia, Colombia;2. Laboratorio de Patología Veterinaria- Facultad de Ciencias Agropecuarias, Universidad de Ciencias Aplicadas y Ambientales (U.D.C.A), Bogotá, Cundinamarca, Colombia;3. Laboratorio Clínico, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Bogotá, Colombia;4. Laboratory of Pharmacology and Toxicology, James Graham Brown Cancer Center, University of Louisville School of Medicine, USA;5. Laboratorio de Patología Veterinaria, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Bogotá, Colombia;1. Department of Biology, University of Naples \"Federico II\", Naples, Italy;2. Laboratory Animal Science (LAS), Institute for Research and Innovation in Health (i3S), University of Porto (UP), Porto, Portugal;3. Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal;4. Inov4Agro, Institute for Innovation, Capacity Building and Sustainability of Agri-food Production, Vila Real, Portugal |
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| Abstract: | Senecio spp. is one of the most frequent plant-related poisonings in cattle. Its ingestion generates the disease seneciosis, characterized by hepatic damages. Liver biopsies and serum markers dosage are tools used in diagnosis; however, many breeding cattle are undiagnosed. MicroRNAs are non-coding RNA, stable in biological fluids. Their difference in expression levels may indicate the presence of the poisoning. We analyzed the miRNA profiling to identify potential diagnostic biomarkers for Senecio brasiliensis poisoning. The expression of miR-21, miR-885, miR-122, miR-181b, miR-30a, miR-378, and let-7 f were evaluated in the serum of exposed cattle. At least one histological change was found in liver and lower quantity of albumin and high AST and ALP were also detected. MiRNAs miR-30a, miR-378, miR-21, miR-885, and miR-122 presented significantly higher expression in intoxicated animals than in healthy animals. Furthermore, miR-122, miR-885, and, especially, miR-21 signatures demonstrated high sensitivity and specificity, with potential application for detecting poisoning. |
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| Keywords: | MiRNA Biomarker Livestock poisoning |
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