|
|||||
|
|
| MHC-I类分子在诱导异体肾癌特异性CTL中的作用 | |
| 引用本文： | 徐龙,马安伦,张冬青,郑志强,葛海良. MHC-I类分子在诱导异体肾癌特异性CTL中的作用[J]. 细胞与分子免疫学杂志, 2001, 17(2): 153-154 |
| 作者姓名： | 徐龙 马安伦 张冬青 郑志强 葛海良 |
| 作者单位： | 1. 上海市杨浦区中心医院检验科 , 上海 200090 2. 上海第二医科大学上海市免疫学研究所 , 上海 200025 |
| 摘 要： | 目的探讨MHC-I类分子匹配的异体人肾癌细胞诱导肾癌细胞特异性CTL的作用。方法用经照射的HLA-I类分子匹配的异体人肾癌细胞作为刺激细胞，诱导外周血CTL，并分析其表型及杀伤效应。结果11例肾癌患者外周血淋巴细胞经HLA匹配的肾癌细胞刺激后，诱导的CTL细胞主要以TCRαβ＋、CD8＋为主，具有明显的杀伤效应。结论用MHC-I类分子匹配的异体肾癌细胞可诱导肿瘤特异性、并具有明显杀伤活性的的CTL。 |
| 关 键 词： | MHC；CTL；肾癌 |
| 文章编号： | 1007-8738(2001)02-153-03 |
| 修稿时间： | 2000-10-06 |
Induction of tumorspecific cytotoxic T lymphocytes from human peripheral blood by stimulation with MHC-Imatched allogenic renal cell carcinoma |
|
| XU Long,MA An lun,ZHANG Dong qing,ZHENG Zhi qiang,GE Hai liangShanghai Second Medical University. Induction of tumorspecific cytotoxic T lymphocytes from human peripheral blood by stimulation with MHC-Imatched allogenic renal cell carcinoma[J]. Chinese journal of cellular and molecular immunology, 2001, 17(2): 153-154 | |
| Authors: | XU Long MA An lun ZHANG Dong qing ZHENG Zhi qiang GE Hai liangShanghai Second Medical University |
| Affiliation: | XU Long2,MA An lun1,ZHANG Dong qing1,ZHENG Zhi qiang2,GE Hai liang11Shanghai Second Medical University,Shanghai Institute of Immunology,Shanghai 200025,2Yangpu District Central Hospital,Shanghai 200090,China |
| Abstract: | Aim To explore the effect of MHC-I matched allogenic renal tumorcells inducing tumor specific cytotoxic T lymphocytes (CTLs). Methods HLA type was defined by HLA serologically test. CTLs were induced by using irradiated known HLA-A allogenic renal tumor cells in the presence of IL-2. Phenotypes of the induced CTLs were analyzed by flow cytometer. The MTT test was used to determine cytotoxic activity of the CTLs. Results CTLs induced by allogenic renal tumor cells from 11 patients were showed to be mainly CD8＋ and TCR-α β ＋ cells and to have specific cytotoxic activity to the MHC-I matched renal cancer cells. Conclusions Renal cancer-specific CTLs may be obtained from PBMC by stimulation with MHC-I matched allogenic renal tumor cells and were showed to have obviously high cytotoxic activity. |
| Keywords: | MHC CTL renal carcinoma |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |