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| 苦参碱和MAPK/ERK信号通路在抑制肺癌细胞诱导人脐静脉内皮细胞增殖和迁移中的作用 | |
| 引用本文： | 卢进昌,罗强,程萍,刘先军,白明,涂明利. 苦参碱和MAPK/ERK信号通路在抑制肺癌细胞诱导人脐静脉内皮细胞增殖和迁移中的作用[J]. 中国肺癌杂志, 2009, 12(7). DOI: 10.3779/j.issn.1009-3419.2009.07.002 |
| 作者姓名： | 卢进昌 罗强 程萍 刘先军 白明 涂明利 |
| 作者单位： | 1. 430030,武汉,华中科技大学同济医学院附属协和医院呼吸内科;442000十堰,郧阳医学院附属太和医院呼吸内科 2. 郧阳医学院附属太和医院呼吸内科,十堰,442000 3. 华中科技大学同济医学院附属协和医院产科,武汉,430030 4. 华中科技大学同济医学院附属协和医院呼吸内科,武汉,430030 |
| 基金项目： | 湖北省教育厅重点项目 |
| 摘 要： | 背景与目的 苦参碱是中国传统中药槐根的主要生物碱成分之一,具有抗炎、抑制肿瘤细胞增殖、诱导肿瘤细胞分化和凋亡等作用.丝裂原活化蛋白激酶/细胞外调节蛋白激酶(Motigen-activated proteinkinase/extraceUuar regulated protein kinase,MAPK/ERK)级联通路是血管内皮细胞信号转导的重要途径.本研究目的 是探讨苦参碱(Matrine,Mat)和MAPK/ERK信号转导通路在抑制肺腺癌A549细胞条件培养基(A549 human lung cancer cellsconditioned medium,A549CM)诱导的人脐静脉内皮细胞(human umbilical veins endothelial cens,HUVECs)增殖和迁移中的作用.方法 应用Mat与MAPK/ERK特异性抑制剂PD98059(PD)处理A549CM培养的HUVECs,采用细胞计数、划痕损伤实验和Western Blot技术,观察Mat、PD98059对A549CM诱导的人脐静脉内皮细胞增殖、迁移及磷酸化ERK(p-ERK)表达的影响.结果 干预后24h,与对照组相比,A549CM显著促进HUVECs增殖、迁移及P-ERK的表达.与A549CM组相比,Mat能明显抑制A549CM诱导的HUVECs增殖和迁移及p-ERK的表达;PD98059可以抑制HUVECs增殖及P-ETK表达,而对迁移没有作用.结论 Mat和PD98059均能有效抑制A549CM诱导的HUVECs增殖和P-ERK(表达,Mat还能抑制A549CM诱导的HUVECs迁移,而PD98059对A549CM诱导的HUVECs迁移没有影响,提示抑制MAPK/ERK(信号传导通路可能是苦参碱抗肺癌血管生成的部分机制. |
| 关 键 词： | 肺肿瘤 苦参碱 丝裂原活化蛋白激酶 细胞外信号调节激酶 血管生成 |
The Role of Matrine and Mitogen-Ativated Protein Kinase/Extracellular Signal-Regulated Kinase Signal Transduction in the Inhibition of the Proliferation and Migration of Human Umbilical Veins Endothelial Cells Induced by Lung Cancer cells |
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| Jinchang LU,Qiang LUO,Ping CHENG,Xianjun LIU,Ming BAI,Mingli TU. The Role of Matrine and Mitogen-Ativated Protein Kinase/Extracellular Signal-Regulated Kinase Signal Transduction in the Inhibition of the Proliferation and Migration of Human Umbilical Veins Endothelial Cells Induced by Lung Cancer cells[J]. Chinese journal of lung cancer, 2009, 12(7). DOI: 10.3779/j.issn.1009-3419.2009.07.002 | |
| Authors: | Jinchang LU Qiang LUO Ping CHENG Xianjun LIU Ming BAI Mingli TU |
| Affiliation: | Jinchang LU1,2,Qiang LUO2,Ping CHENG3,Xianjun LIU2,Ming BAI1,Mingli TU2 1Department of Respiratory Medicine,Union Hospital,the First Affiliated Hospital of Tongji Medical College,Huazhong Science and Technology University,Wuhan 430030,China,2Department of Respiratory Medicine,3Department of Obstetrics,Taihe Hospital,the First Affiliated Hospital of Yunyang Medical College,Shiyan 442000 |
| Abstract: | Background and objective Matrine,one of the major alkaloid components of the traditional Chinese medicine Sophora roots,has a wide range of pharmacological effects including anti-inflammatory activities,growth inhibition and induction of cell differentiation and apoptosis.Motigen-activated protein kinase(MAPK)/extracellular signal-regulated kinase(ERK) has found to be a crucial signaling pathway in endothelial cells.The aim of this study is to investigate the role of Matrine and MAPK/ERK signal transduction... |
| Keywords: | Lung neoplasms Matrine MAPK ERK Angiogenesis |
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