应用Triton-x100加丹参酚酸B制备脱细胞血管支架及其血液相容性研究

应用传统的Triton-x100法与应用Triton-x100和丹参酚酸B法制备脱细胞血管支架,比较这两种支架的理化性质及其血液相容性,展望应用丹参酚酸B后处理脱细胞血管支架在临床上治疗心脑血管疾病方面的应用前景,以及在组织工程血管材料方面的开发空间。选择SD大鼠16只,雄性,鼠龄7周,体质量250 g左右。解剖大鼠,取腹主动脉;随机分为2组,每组8个。用Triton-x100法(Tx组)、Triton-x100加入丹参酚酸B法(Tx-sal 组),加工制成两种脱细胞血管支架。观察脱细胞血管支架的宏观形貌;亲水性实验测定亲水性;体外溶血实验计算体外溶血率;复钙化凝血时间实验测定复钙化凝血时间;血小板黏附实验测定动态凝血时间,以及体外血小板的黏附;补体激活实验测定补体激活程度情况;比较两组脱细胞血管支架的血液相容性。Tx组接触角为76.36°±4.65°,Tx-sal组接触角为71.26°±3.55°,Tx组接触角度数略高于Tx-sal 组,差异无显著统计学意义。Tx-sal 组溶血率为1.5%,Tx组溶血率为2.1%。Tx组复钙化凝血时间为(212±11.32)s,Tx-sal 组复钙化凝血时间为(231±13.53)s,Tx组与Tx-sal 组的复钙化凝血时间差异有显著统计学意义。Tx-sal组相比Tx组延缓了凝血时间,抗凝血性更加优良。Tx-sal 组黏附的血小板数目低于Tx组的相应数目。Tx-sal 组补体激活水平低。与传统的Triton-x100法制备的脱细胞血管支架相比,加入丹参酚酸B后处理的脱细胞血管支架具有更加优良的血液相容性,更适合作为组织工程材料应用于心脑血管疾病的治疗,在临床医学上的应用前景更加广阔。

Study on the Preparation and Blood Compatibility of Acellular Vascular Scaffold with Triton-x100 and Salvianolic Acid B

To compare the physicochemical properties and blood compatibility of two kinds of acellular vascular stents prepared by traditional Triton-x100 method and salvianolic acid B with Triton-x100. To explore the prospect of the application of salvianolic acid B post-treated acellular vascular stents in the treatment of cardiovascular and cerebrovascular diseases and the development of tissue engineering vascular materials. A total of 16 SD rats, male and 7 weeks old, with a mass of 250 g, were included and randomly divided into 2 groups, 8 in each group. Two kinds of acellular vascular scaffolds prepared by Triton-x100 method (Tx group) and Triton-x100 adding salvianolic acid B method (Tx-sal group) were implanted. The macroscopical morphology, hydrophilicity, in vitro hemolysis rate and the determination of coagulation time of complex calcification were investigated. The blood compatibility of two groups of acellular scaffold was compared on the basis of a series of experiments. The contact angle of Tx group was (76.36±4.647), Tx-sal group was (71.26±3.553), and Tx group was slightly higher than Tx-sal group, which didn′t have significant difference in statistics. Hemolysis rate in the group of Tx-sal was 1.5% and hemolysis rate in the group of Tx was 2.1%. The coagulation time of recalcification in Tx group was (212±11.32) s, and that of Tx-sal group was (231±13.53) s. There was significant difference in the coagulation time between Tx group and Tx-sal group. Group Tx-sal had delayed coagulation time and had better anticoagulation when compared with group Tx. The platelet count in the group of Tx-sal was lower than that in the group of Tx. The level of complement activation was lower in the group of Tx-sal. In conclusion, the acellular scaffold with salvianolic acid B had better blood compatibility than the acellular scaffold prepared by traditional Triton-x100 method.