甘草提取液对糖尿病大鼠肾脏的保护作用

目的 观察甘草提取液对糖尿病大鼠肾脏的保护作用及其机制。方法 Wistar大鼠48只随机分为对照组、模型组和治疗组，每组16只。于第8周及第12周测定各组大鼠的肾质量指数（KI）、葡萄糖氧化酶法测定空腹血糖（FBG）、微柱法测定糖化血红蛋白（GHbA1c）、考马斯亮兰法测定24h尿白蛋白量、速率法测定血和尿肌酐、硫代巴比妥酸法测定血液及肾组织匀浆中丙二醛（MDA）含量、黄嘌呤氧化酶法测定血液及肾组织匀浆中超氧化物歧化酶(SOD)活性、比色法测定血液及肾组织匀浆中硒谷胱甘肽过氧化物酶（SeGSHPx）和醛糖还原酶（AR）活性、计算内生肌酐清除率（Ccr）。结果 与对照组相比，模型组及治疗组所有指标的差异均有统计学意义（P均＜0.05）。与模型组相比，治疗组KI、FBG、GHbA1c、24h尿白蛋白量、Ccr、血液及肾组织匀浆中MDA含量、AR活性均显著降低（P均＜0.05），SOD、SeGSHPx活性均显著升高（P均＜0.05）。结论 甘草提取液对糖尿病大鼠具有肾脏保护作用，其机制与调节血糖、降低肾脏AR活性、提高肾脏抗氧化能力有关。

Renal protective effects of licorice extracts in diabetic rats

Objective To investigate the protective effects of licorice extracts on the kidneys of diabetic rats and its mechanism.Methods 48 Wistar rats were randomly divided into 3 groups: the control group,the diabetic group and the therapy group.There were 16 rats in each group.At the 8th,12th week,KI was detected,FBG was detected by the glucose-oxidase method,GHbAlc was detected by the micro-column method,24 h urinary albumin was detected by the Coomassie brilliant blue method,urine creatinine and serum creatinine...