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| 人转化生长因子β3的构建 | |
| 引用本文： | 张世平,卢日峰,刘晶珠,陈强,凌翎,李鹏,田亚萍. 人转化生长因子β3的构建[J]. 吉林大学学报(医学版), 2006, 32(4): 565-567. DOI: 国家自然科学基金资助课题（30070705）；中 |
| 作者姓名： | 张世平 卢日峰 刘晶珠 陈强 凌翎 李鹏 田亚萍 |
| 作者单位： | 吉林大学中日联谊医院预防保健科,吉林,长春,130033;吉林大学公共卫生学院毒理学教研室,吉林,长春,130021;吉林大学第二医院妇产科,吉林,长春,130041;吉林大学第一医院皮肤科,吉林,长春,130021 |
| 基金项目： | 国家高技术研究发展计划(863计划) , 中华医学会基金 |
| 摘 要： | 目的:获取人转化生长因子β3的编码基因，开发抗衰老的皮肤药物。方法：采用重叠PCR的方法，设计4对引物，进行4次PCR合成人转化生长因子β3的编码基因。结果：4次PCR后，经2％的琼脂糖凝胶电泳可见一357 bp的条带，将电泳产物回收，连接入pMD-18T载体，经测序分析证实，所获得DNA片段为人转化生长因子β3的编码基因。结论：利用重叠PCR方法能够成功构建人转化生长因子β3的编码基因。 |
| 关 键 词： | 转化生长因子 皮肤衰老 紫外线 聚合酶链反应/方法 |
| 文章编号： | 1671-587X(2006)04-0565-03 |
| 收稿时间： | 2005-11-25 |
| 修稿时间： | 2005-11-25 |
Construction of human transforming growth factor-beta 3 |
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| ZHANG Shi-ping,LU Ri-feng,LIU Jing-zhu,CHEN Qiang,LING Ling,LI Peng,TIAN Ya-ping. Construction of human transforming growth factor-beta 3[J]. Journal of Jilin University: Med Ed, 2006, 32(4): 565-567. DOI: 国家自然科学基金资助课题（30070705）；中 | |
| Authors: | ZHANG Shi-ping LU Ri-feng LIU Jing-zhu CHEN Qiang LING Ling LI Peng TIAN Ya-ping |
| Affiliation: | 1. Department of Preventive Health Care, China-Japan Union Hospital, Jilin University, Changchun 130033,China；2. Department of Toxicology, School of Public Health, Jilin University, Changchun 130021, China；3. Department of Obstetrics and Gynecology,Second Hospital, Jilin University, Changchun 130041, China；4. Department of Dermatology, First Hospital, Jilin University, Changchun 130021, China |
| Abstract: | Objective To obtain the encoding gene of human transforming growth factor-beta 3 in order to develop an effective antiaging skin drugs.Methods The overlaying PCR was used to acquire human transforming growth factor-beta 3.Results A 357 bp DNA band was seen in the 2% agarose gel after the PCR product was electrophoresed.After the 357 bp DNA band was recovered and ligated into pMD-18T,human transforming growth factor-beta 3 encoding gene acquired through four-cycle PCR was confirmed by sequencing.Conclusion Human transforming growth factor-beta 3 encoding gene is successfully constructed by overlaying PCR. |
| Keywords: | transforming growth factors skin aging ultraviolet ray polymerase chain reaction/methods |
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