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| 参芎葡萄糖注射液对缺血再灌注损伤心肌的保护作用 | |
| 引用本文： | 李达文,龚惠,叶勇,路静,蒋国良,贾剑国,吴超能,张国平,邹云增. 参芎葡萄糖注射液对缺血再灌注损伤心肌的保护作用[J]. 中国分子心脏病学杂志, 2012, 12(1): 51-56 |
| 作者姓名： | 李达文 龚惠 叶勇 路静 蒋国良 贾剑国 吴超能 张国平 邹云增 |
| 作者单位： | 1. 复旦大学生物医学研究院, 上海,200032 2. 同济大学东方转化医学研究中心, 上海,200120 3. 200032 上海,复旦大学附属中山医院,上海市心血管病研究所 4. 200032 上海,复旦大学生物医学研究院;200032 上海,复旦大学附属中山医院,上海市心血管病研究所 |
| 基金项目： | 国家自然基金重点项目(30930043);国家自然基金青年基金项目(81000041);上海市重点专项课题(08dj1400504) |
| 摘 要： | 目的探讨参芎葡萄糖注射液对小鼠急性心肌缺血再灌注（I／R）损伤的保护作用及可能的分子机制。方法建立急性心肌缺血再灌注（Ischemia-Reperfusion，I／R）损伤的小鼠模型，将野生型小鼠（C57BL／6）随机分为3组，假手术组（Sham），缺血再灌注＋生理盐水组（Vehicle），缺血再灌注＋参芎葡萄糖注射液预处理组（20mL／kg，SL），术前2小时给予腹腔注射生理盐水或参芎葡萄糖注射液，于缺血再灌注后不同时间点，通过心脏超声观察各组小鼠心功能的变化，心肌组织TTC染色确定心梗面积大小，脱氧核糖核苷酸末端转移酶法（TUNEL）检测凋亡心肌，蛋白免疫印迹法（WesternBlot）及酶联免疫吸附法（ELISA）分别检测心肌组织以及血清炎症因子肿瘤坏死因子（TNF-α）表达水平变化。结果I／R手术48小时后，与假手术组相比，左室射血分数（LVEF）明显降低（P〈0．01）。参芎葡萄糖注射液预处理明显改善I／R组的LVEF，并降低了心肌梗死面积，抑制I／R引起的心肌细胞凋亡和组织炎症反应。进一步的研究表明，参芎葡萄糖注射液预处理明显抑制了I／R后心肌组织以及血清中TNF-α表达水平升高（P〈0．01）。结论参芎葡萄糖注射液预处理通过抑制TNF-α表达水平发挥对缺血再灌注（I／R）损伤心肌的保护作用。 |
| 关 键 词： | 参芎葡萄糖注射液 心肌缺血再灌注损伤 炎症反应 细胞凋亡 |
The cardio-protection of Salvia miltiorrhiza Ligustrazine Hydrochloride and Glucose Injection on myocardial ischemia-reperfusion injury in mice |
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| LI Da-Wen , GONG Hui , YE Yong , LU Jing , JIANG Guo-Liang , JIA Jian-guo , WU Chao-Neng , ZHANG Guo-Ping , ZOU Yun-Zeng. The cardio-protection of Salvia miltiorrhiza Ligustrazine Hydrochloride and Glucose Injection on myocardial ischemia-reperfusion injury in mice[J]. Molecular Cardiology of China, 2012, 12(1): 51-56 | |
| Authors: | LI Da-Wen GONG Hui YE Yong LU Jing JIANG Guo-Liang JIA Jian-guo WU Chao-Neng ZHANG Guo-Ping ZOU Yun-Zeng |
| Affiliation: | 1,2(1.Research Center for Cardiovascular Diseases,Institutes of Biomedical Sciences,Fudan University,Shanghai,200032,China;2.Shanghai Institute of Cardiovascular Diseases,Zhongshan Hospital,Fudan University,Shanghai 200032,China;3.Research Center for Translational Medicine,Shanghai East Hospital,Tongji University,Shanghai 200120,China) |
| Abstract: | Objective To investigate the cardio-protective effect of Salvia miltiorrhiza Ligustrazine Hydrochloride and Glucose injection (SL) induced by ischemic pre-conditioning (IPC) following myocardial ischemia-reperfusion (I/R) injury in mice.Methods I/R injury model was obtained by ligating the proximal left anterior descending coronary artery 30 min followed by different hours reperfusion.Wild-type C57BL/6 mice were randomly divided into 3 groups and separately subjected to IPC treatments:Sham control group;I/R+Vehicle control group;I/R+SL group (20 mL/kg);The mice were treated with the SL by peritoneal injection 2 hours before the surgery.Cardiac function was detected by echocardiography,TTC staining was performed to evaluate the area of myocardial infarction,The number of apoptotic cardiomyocyte was detected by TUNEL assay,TNF-α levels in serum and myocardium was measured by ELISA and Western Blot,respectively.Results Cardiac function of mice in SL group were greatly improved at 48 hours after I/R surgery (P<0.01).Further research indicated that SL pretreatment significantly reduced infarct size,inhibited apoptosis,attenuated inflammation response in myocardium,and decreased TNF-α level in serum and myocardium,compared with the vehicle group (P<0.01).Conclusion "Salvia miltiorrhiza Ligustrazine Hydrochloride and Glucose injection" exerts cardio-protective effects on ischemia-reperfusion injury by inhibiting TNF-α level. |
| Keywords: | Salvia miltiorrhiza Ligustrazine Hydrochloride and Glucose injection Myocardial ischemia-reperfusion injury Inflammatory Response,Cardiomyocyte Apoptosis. |
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