硒化壳聚糖诱导NB4细胞凋亡过程中下调了survivin的表达

目的:研究硒化壳聚糖对NB4细胞凋亡及周期阻断作用,探讨这种作用与survivin蛋白表达的关系。方法:细胞分为三个浓度组(空白对照组、50mg/L组、100mg/L组),激光共聚焦显微镜和流式细胞仪观察药物对细胞凋亡及周期阻断作用;Western blot法检测survivin蛋白含量的变化。结果:硒化壳聚糖可诱导NB4细胞凋亡并阻断细胞于G0G1期,还可降低细胞survivin蛋白含量。结论:硒化壳聚糖可通过下调NB4细胞survivin蛋白含量,进而诱导细胞发生凋亡。

The inducing apoptosis effect of selenium chitosan on NB4 cells correlates with down regulation of survivin protein.

Objective： TO Study the inducing apoptosis and cell cycle block effect of selenium chitosan on NB4 ceils and to explore its relationship with the survivin protein. Methods ： The inducing apoptosis and cell cycle block effect was observed by using flow cytometry and Laser confocal scan microscope. Western blotting was used to examine the abundance of survivin protein expressed by tumor cells. Results： Exposure of NB4 cells to 50-100mg./L of selenium chitosan for 24h produced the increase in G0--G1 phase cells and the apoptosis rate as well. The abundance of survivin protein was strongly dpwn regulated in 100 mg./L selenium chitosan treated NB4 cells. Conclusions： Selenium chitosan can induce NB4 cells apoptosis and block NB4 cells in GO G1 phase by downing the regulation of the abundance of survivin protein .