他莫昔芬和莪术油联合治疗大鼠子宫内膜异位症的实验研究

目的观察选择性雌激素受体调节剂他莫昔芬（TAM）与中药促细胞凋亡剂（莪术提取物）联合用药对大鼠子宫内膜异位症的治疗效果。方法建立雌性大鼠子宫内膜异位症模型，随机分为对照组，TAM、阿拉瑞林（GnRHa）和TAM＋莪术油治疗组，治疗4周后观察异位内膜生长情况，切取在位与异位子宫内膜，行HE染色观察组织形态学变化，SP免疫组化法检测异位内膜血管内皮生长因子（VEGF）的表达。结果各治疗组治疗后异位内膜体积较治疗前及对照组减小（P〈0．05）；治疗后各治疗组异位内膜的VEGF表达评分较对照组少，其差异有统计学意义（P〈0．05）；治疗后TAM组与阿拉瑞林（GnRHa）组、TAM组与TAM＋莪术油组异位内膜VEGF表达评分差异有统计学意义（P〈0．05）。结论TAM结合莪术油可使大鼠异位内膜病灶缩小，病灶VEGF表达降低。

Experimental Study on Rat Model of Endometriosis Treated with Tamoxifen and Rhizoma Curcumae Oil

Objective To evaluate the therapeutic effect of Tamoxifen in conjunction with a cell apoptosis contributor-Rhizoma curcumae oil on the rat endometriosis models.Methods The rat endometriosis models were established and were randomly assigned into the control group,Tamoxifen group,Alarelin(GnRHa) group,and the group of Tamoxifen(by gastrogavage) plus Rhizoma curcumae oil(by intraperitoneal injection).After 4 weeks of treatment,the growth of ectopic endometrial implants was observed;then eutopic and ectopic endometria were excised in preparation for HE staining and streptavedin-peroxi-dase immunohisto chemical analysis.Results After treatment,the volume of the ectopic implants of each treatment group was markedly smaller than that of control and before treatment(P<0.05);the score of VEGF showed significant difference when each treatment group was compared with control(P<0.05).The score of VEGF exhibited marked difference between Tamoxifen group and Alarelin(GnRHa) group,between Tamoxifen group and Tamoxifen plus Rhizoma curcumae oil group(P<0.05).Conclusion Tamoxifen in conjunction with Rhizoma curcumae oil could decrease markedly the volume and VEGF expression of ectopic implants.