解毒通络保肾法抑制糖尿病肾病炎症发病机制的研究

目的:研究中药解毒通络保肾胶囊对糖尿病(DM)大鼠肾脏病变的保护作用及其机制。方法:将实验动物分为正常对照组、DM组、解毒通络保肾胶囊中药实验组和苯那普利阳性对照组。检测各组第12周的血糖、尿素氮、血肌酐、尿白蛋白排泄率(UAER)、血脂、血管紧张素Ⅱ(AngⅡ)含量和肾脏肥大指标的变化,应用RT-PCR检测MCP-1mRNA,免疫组化检测肾内纤维连接蛋白(FN)和Ⅳ型胶原(ColⅣ)蛋白、PCNA蛋白、NF-κBp65蛋白、MCP-1蛋白、ED-1蛋白表达水平。结果:中药实验组较DM组血糖水平显著降低(P<0.01),UAER明显减少(P<005),尿素氮和血肌酐水平下降,肾脏肥大指标改善(P<0.01);明显降低AngⅡ含量,PCNA、FN和ColⅣ蛋白表达显著低于DM组(P<001)。RT-PCR结果表明,DM大鼠肾皮质MCP-1mRNA表达升高,中药实验组MCP-1mRNA低于DM组(P<0.05)。结论:解毒通络保肾胶囊对DM肾脏病变有保护作用,其机制可能是通过减少肾组织中AngⅡ含量,下调DM大鼠NF-kB,减少MCP-1表达,从而抑制肾内炎症的反应。

Study on the Protective Effects of JieDuTongLuoBaoShen Capsules Against Renal Lesion in Diabetic Rats

Objective：To investigate the protective effects of JieDuTongLuoBaoShen Capsules （JC） against renal lesion in diabetic rats.Method：The experimental animals were divided into normal control group,diabetic control group,posivite control group and experimental group.The level of the blood sugar,urea nitrogen blood creatinine,angiotensin Ⅱ（AngⅡ）were measured and the excretion rate （UAER） of urinary albumin and index of renal hypertrophy were detected in each group at the 12th week after the experiment.At the same time,RT-PCR was used to detect the mRNA expression of renal monocyte chemoattractant protein-1 （MCP-1）,and immunohistoche mical techniques were used to detect the expressions of renal fibronectin （FN）,type Ⅳ collagen （Col Ⅳ）, proliferating cell nuclear antigen （PCNA）, nuclear factor Kappa B（NF-κB）,MCP-1, macrophage mark antigen（ED-1）.Results：In the JC-treated group,the blood sugar level was markly lowered （P〈0.01）,UAER was markedly reduced（P〈0.05）, the levels of urea nitrogen and blood ereatinine were decreased,and the renal hypertrophy index was improved （P 〈0.01）,as compared with those in the diabetic control group.The RT-PCR detection showed that the mRNA expression ofreaal MCP-1 was increased （P 〈0.05）,and the sediment ofglomerular FN ,Col Ⅳ,PCNA,NF-κB,MCP-1 and ED-lwere lower than those in diabetic control group （P 〈0.01）.Conclusion：The mechanism of the protective effects of JC against renal lesion may be decreasing the levels of Angll in the renal tissue ,downregulating the level of NF-κB,reducing the expression of MCP-1,and inhibiting the inflammatory pathogenesis of diabetic nephropathy.