炎症因子及益肾活血泄浊汤对大鼠肾小球系膜细胞生长的影响

目的：探讨部分炎症因子及益肾活血泄浊汤对体外培养大鼠肾小球系膜细胞生长的影响。方法：应用脂多糖（ＬＰＳ）及白细胞介素６（ＩＬ－６）作为刺激因子，观察其促系膜细胞增殖的作用；并采用血清药理学方法，提取动物的含药血清作用于一述受刺激的系膜细胞，观察益肾活血泄浊汤对系膜细胞增殖的影响。结果：ＬＰＳ和ＩＬ－６具有明显促进系膜细胞增殖作用。结论：系膜细胞是益肾活泄浊汤发挥治疗作用的重要靶细胞，这可能是该方防

Effect of Inflammatory Factor and Yishen Huoxue Xiezhuo Decoction on Growth of Glomerular Mesangial Cells in Rats

To explore the part of inflammatory factors (cytokines) and Yishen Huoxue Xiezhuo (YSHXXZ) Decoction on the proliferation of extracorporeal cultured mesangial cells (MCs) in rats. Methods: The effects of lipopolysaccharide (LPS) and interleukin 6 (IL 6) as stimulating factors, its action on the proliferation of rat MCs were investigated by using the technique of 3 H TdR incorporation. Meanwhile, adopting serum pharmacology assay, the medicated serum of rat, containing YSHXXZ decoction was extracted and its effects on the growth of MCs were also studied. Results: LPS and IL 6, in a dose dependent and time dependent manner, could markedly stimulate the MCS proliferation, while this stimulatory effects could be strongly antagonized by the serum containing YSHXXZ decoction. Conclusion: Mesangial cell is the main target cell of the action of YSHXXZ decoction, and the inhibition on MCs might be one of the mechanisms of the YSHXXZ decoction in preventing the progression of chronic glomerulonephritis.