程序升温毛细管气相色谱的精油成分保留指数研究

本文采用线性程序升温毛细管气相色谱法对200多种天然存在和人工合成的挥发油、香精成分,分别在极性柱(DB-WAX)和非极性柱(DB-1)上进行了Kovats保留指数的测定。归纳了部分化合物的保留指数规律;考察了气体平均线速,程序初温及升温速率对保留指数的影响。并结合我们所开发的色谱数据库管理系统进行了香精的定性分析。

RETENTION INDEX OF ESSENTIAL OIL IN TEMPERATUREPROGRAMMED CAPILLARY COLUMN GAS CHROMATOGRAPHY

Kovats retention indexes of more than 200 kinds of natural and synthetic compounds of alcohol, aldehyde, acetal, ketone, ether, alkene, nitrile, carboxylic acid, ester, etc. were determined on polar liquid phase (DB-WAX) and nonpolar liquid phase (DB-1) by linear temperature-program capillary column gas chromatography. Some rules of part of the homologous series of compounds were summed up from the retention indexes: the rules of RI vs carbon, RI vs boiling point and RI on two columns. In order to keep the retention indexes practical, effects of initial temperature (T0), program rate (r) and average linear carrier gas velocity (u), etc. on the retention indexes were investigated. The conclusion is that the retention indexes are highly reproducible so long as the program parameters change in a certain range. Two kinds of essences, Meigui and Linglan, were identified successfully on IBM-PC/XT microcomputer by the Data Base Management System of essential oil and essence by capillary column gas chromatography and the results are satisfactory.