| 高效液相色谱法检测血清帕罗西汀药物浓度 |
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| 引用本文： | 林治光,翁毅仁,庄冬梅,蒋继承,李华芳,殷金龙,王克勋,顾牛范. 高效液相色谱法检测血清帕罗西汀药物浓度[J]. 上海精神医学, 2002, 14(2): 71-73 |
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| 作者姓名： | 林治光 翁毅仁 庄冬梅 蒋继承 李华芳 殷金龙 王克勋 顾牛范 |
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| 作者单位： | 200030,上海市精神卫生中心;200030,上海市精神卫生中心;200030,上海市精神卫生中心;200030,上海市精神卫生中心;200030,上海市精神卫生中心;200030,上海市精神卫生中心;200030,上海市精神卫生中心;200030,上海市精神卫生中心 |
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| 摘 要： | 目的建立一种简便、快速、灵敏的反相高效液相色谱(HPLC)紫外检测法测定血清帕罗西汀药物浓度。方法选用C18柱,波长295nm,丙咪嗪为内标,移动相为甲醇：水：三氯甲烷：异丙醇：四甲基乙二胺：冰乙酸=282：140：1．6：0．8：1．6：2,流速1．0ml／min,以甲苯、正己烷(1：4)为萃取液。结果帕罗西汀和内标的保留时间分别为3．40min和4．20min,批内和批间平均回收率各为98．4％～100．0％和98．8％～101．8％,RSD分别小于8．0％和5．1％,最低有效检测浓度为3ng／ml。结论本法操作简便、快速而精确,具有一定的实用价值。
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| 关 键 词： | 高效液相色谱 帕罗西汀 |
Determination fof paroxetine concetration in high performance liquid chromatography |
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| Lin Zhiguang,Weng Yiren,Zhuang Dongmei,et al.. Determination fof paroxetine concetration in high performance liquid chromatography[J]. Shanghai Archives of Psychiatry, 2002, 14(2): 71-73 |
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| Authors: | Lin Zhiguang Weng Yiren Zhuang Dongmei et al. |
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| Affiliation: | Lin Zhiguang,Weng Yiren,Zhuang Dongmei,et al. Shanghai Mental Health Center,Shanghai 200030 |
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| Abstract: | Objectives: To develop a simple and rapid HPLC method for the determination of the blood level of Paroxetine. Method: The analysis involved a C18 column as stationary phase and methanol. distilled water, isopropanol, trichloromethane, TMEDA, HAc as mobile phase. The flow rate was 1. 0 ml/min-1, UV detection wavelength was 295 mm, Imipramine was used as internal standard. The serum samples were extracted with toluene and n-hexane. Results: Intra-day and inter-day RSD were less than 8. 0% and 5. 1% respectively the recovery rates of intra-day RSD were less than 8. 0% and 5. 1% respectively the recovery rats of intra-day and inter-day were 98. 4%-100. 0% and 98. 8%-101. 8% respectively. Quantification limits were 3 ng/ml. Conclusion: The method is simple, rapid, sensitive and accurate for clinical practice. |
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| Keywords: | HPLC Paroxetine |
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