牙本质磷蛋白mRNA原位杂交方法的建立

目的:建立检测牙本质磷蛋白(DPP)mRNA表达的原位杂交方法。方法:选用发育各阶段的牙胚、牙齿和体外培养的MDPC-23成牙本质细胞为对象,采用地高辛标记的寡核苷酸探针的原位杂交方法。结果:DPP mRNA在牙胚与牙齿中的成牙本质细胞、前成釉细胞和体外培养的成牙本质细胞存在阳性表达。结论:设计的探针敏感性高,特异性高,所建立的原位杂交方法是研究牙本质发育和损伤修复的良好方法。

Establishing of in situ hybridization method for the expression of dentin phosphoprotein mRNA

Objective:To establish method of in situ hybridization for the expression of dentin phosphoprotein. Method:Developing tooth germ or teeth and in vitro cultured mouse MDPC-23 odontoblasts were selected. Oligonucleotide probe labeled with digoxigenin were used in in situ hybridization. Result:DPP mRNA could be detected in the odontoblast and preameloblasts in developing tooth germ or teeth and in vitro cultured mouse MDPC-23 odontoblasts. Conclusion:The DPP oligonucleotide probe we designed showed sensitivity and specificity. In situ hybridization method we established were a good method to study on tooth development and pulp repair after injury.