| 中心组合设计——响应面法优化猪苓多糖提取工艺 |
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| 引用本文： | 魏学军,林先燕,李琼兰,何顺莉,李泽雄,刘亚军.中心组合设计——响应面法优化猪苓多糖提取工艺[J].黔南民族医专学报,2011,24(3):164-167. |
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| 作者姓名： | 魏学军 林先燕 李琼兰 何顺莉 李泽雄 刘亚军 |
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| 作者单位： | 黔南民族医学高等专科学校,贵州都匀,558003 |
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| 基金项目： | 黔南民族医学高等专科学校科研基金资助项目(QNYZ200902) |
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| 摘 要： | 目的：优化猪苓多糖提取工艺。方法：以猪苓中多糖得率为响应值,采用中心组合设计（CCD）-响应面法（RSM）对其提取工艺进行研究。结果：最优工艺参数为液固比11：1,醇沉浓度77%,提取温度95℃,提取时间2.73 h,提取2次。结论：中心组合设计-响应面法可在连续范围内进行分析,具有实验周期短、精密度高的优点。优化结果可为制剂生产提供参考依据。
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| 关 键 词： | 猪苓 多糖 中心组合设计 响应面法 |
Optimize polysaccharide extracting process of Polyorus by central composite design and response surface method |
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| WEI Xue-jun,LIN Xian-yan,LI Qiong-lan,HE Shun-li,LI Zhe-xiong,LIU Ya-jun.Optimize polysaccharide extracting process of Polyorus by central composite design and response surface method[J].Journal of Qiannan Medical College for Nationalities,2011,24(3):164-167. |
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| Authors: | WEI Xue-jun LIN Xian-yan LI Qiong-lan HE Shun-li LI Zhe-xiong LIU Ya-jun |
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| Institution: | WEI Xue-jun,LIN Xian-yan,LI Qiong-lan,HE Shun-li,LI Zhe-xiong,LIU Ya-jun(Qiannan Medical College for Nationalities,Duyun 558003,China) |
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| Abstract: | Objective： Optimize polysaccharide extracting process of Polyorus. Methods： To polyorus polysaccharide rate for response, to study the extracting process by central composite design （CCD） and response surface method （RSM） . Results： The best parameters ： the ratio of solvent to material is 11 ： 1, ethanol concentration is 77%, extracting temperature 95 ℃, the time of extraction is 2. 73 h and the extracting 2 times. Conclusion： Central composite design and response surface method in continuous range analysis, with experimental cycle is short, the advantages of high precision. The optimization results can provide reference basis for production. |
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| Keywords: | Polyporus umbellatus Polysaccharide Central composite design Response surface method |
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