东莞地区已婚育龄妇女人乳头状瘤病毒感染因素及分布情况

目的 探讨东莞地区已婚育龄妇女人乳头状瘤病毒（HPV）感染的相关因素及其亚型分布特点.方法 对4 332名生育年龄女性采用保密式问卷调查;采用PCR反向点杂交法进行HPV16、HPV 18、HPV 31、HPV 33、HPV 35、HPV 39、HPV 45、HPV 51、HPV 52、HPV 53、HPV 56、HPV 58、HPV 59、HPV 66、HPV 68、CP8304、HPV 6、HPV 11、HPV 43 19种宫颈HPV病毒亚型的分型检测并同时进行液基薄层细胞学检查.结果 4 332名中感染育龄妇女中检测HPV亚型398例,总感染率为9.19%,高危感染率为7.55%,排在前5位的高危亚型是HPV16（2.216%）、HPV 58（1.293%）、HPV 52（0.877%）、HPV 53（0.646%）、HPV 33（0.600%）;单一感染340例（85.43%）,二重感染49例（9.88%）,三重感染5例（1.01%）,四重感染4例（0.815%）,组间比较差异有统计学意义（P 〈 0.05）.结论 HPV感染与年龄、职业、性生活频率、文化程度、过早有性生活、孕次等相关.文化程度较低的妇女HPV感染机率较高,有必要加强对文化程度较低妇女的卫生保健知识宣传,从而预防HPV感染,防止宫颈癌的发生.

Infection factors and distribution of human papilloma virus in married women of childbearing age in Dongguan area

Objective To explore the related factors and its subtype distribution characteristics of human papilloma virus(HPV) infection in married women of childbearing age in Dongguan area.Methods 4 332 married women of childbearing age were surveyed by confidentiality-style questionnaire.PCR-reverse dot blot hybridization method was adopted to detect 19 kinds of cervical HPV virus subtype,including HPV 16,HPV 18,HPV 31,HPV 33,HPV 35,HPV 39,HPV 45,HPV 51,HPV 52,HPV 53,HPV 56,HPV 58,HPV 59,HPV 66,HPV 68,HPV CP8304,HPV 6,HPV 11,HPV 43,at the same time liquid-based thin-layer cytology check was adopted.Results Among 4 332 married women of childbearing age,subtype HPV infection was detected in 398 cases,the total infection rate was 9.19%,high-risk infection rate was 7.55%.The high-risk subtypes standing in the front fifth place were HPV 16(2.216%),HPV 58(1.293%),HPV 52(0.877%),HPV 53(0.646%),HPV 33(0.600%).Single infections were in 340 cases(85.43%),double infections were in 49 cases(9.88%),three overlapping infections were in 5 cases(1.01%),and four overlapping infection were in 4 cases(0.815%),the differences among the groups were statistically significant(P < 0.05).Conclusion HPV infection associated with age,occupation,frequency of sexual life,education level,early sexual life and pregnancy number.The HPV infection of women with low educational level is higher,it is necessary to strengthen the health knowledge propaganda in low educational level women,in order to prevent HPV infection and the incidence of cervical cancer.