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| HPLC法测定气血双补口服液中芍药苷的含量 | |
| 引用本文： | 汤淏,崔恩忠,唐安福,王曙东. HPLC法测定气血双补口服液中芍药苷的含量[J]. 中国医药导报, 2012, 9(14): 127-128 |
| 作者姓名： | 汤淏 崔恩忠 唐安福 王曙东 |
| 作者单位： | 1. 南京中医药大学,江苏南京,210046 2. 南京军区南京总医院,江苏南京,210002 |
| 摘 要： | 目的建立气血双补口服液中芍药苷含量的测定方法,为提高该制剂的质量标准提供研究依据。方法采用高效液相色谱法,流动相：甲醇-0.1%磷酸（29∶71）;色谱柱：汉邦ODS-C18柱（250 mm×4.6 mm,5μm）;体积流量：1.0mL/min;柱温：室温;检测波长：230 nm;进样量：10μL。结果芍药苷在10.00～80.00μg/mL范围内线性关系良好,平均回收率为99.45%,相对标准偏差（RSD）为0.63%。结论该方法简便、可靠、重现性好,可用于气血双补口服液的质量控制。 |
| 关 键 词： | 气血双补口服液 芍药苷 高效液相色谱法 |
HPLC determination of Paeoniflorin in Qixueshuangbu Oral Liquid |
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| TANG Hao , CUI Enzhong , TANG Anfu , WANG Shudong. HPLC determination of Paeoniflorin in Qixueshuangbu Oral Liquid[J]. China Medical Herald, 2012, 9(14): 127-128 | |
| Authors: | TANG Hao CUI Enzhong TANG Anfu WANG Shudong |
| Affiliation: | 1.Nanjing University of Traditional Chinese Medicine,Jiangsu Province,Nanjing 210046,China;2.Nanjing General Hospital of Nanjing Military Area Commands,Jiangsu Province,Nanjing 210002,China |
| Abstract: | Objective To establish a method for the determination of Paeoniflorin in Qixueshuangbu Oral Liquid,which could provide envidence of research for quality standard.Methods HPLC method was used with Hanbang ODS-C18 column(250 mm × 4.6 mm,5 μm) and a mobile phase of methanol-0.1% phosphoric acid solution(29∶71).Flow rate was 1.0 mL/min,column temperature was 25℃,the detection wavelength was setted at 230 nm and sample loop volume was 10 μL.Results The calibration curve was linear within the range of 10.00-80.00 μg/mL of Paeoniflorin,the average recovery was 99.45%(RSD=0.63%).Conclusion The method is simple,accurate and repeatable,which can be used for the quality control of Qixueshuangbu Oral Liquid. |
| Keywords: | Qixueshuangbu Oral Liquid Paeoniflorin HPLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |