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| 小鼠成釉蛋白基因cDNA全序列的克隆 | |
| 引用本文： | 顾淑萍,史俊南,刘晗,郝建军,汪平,费俭. 小鼠成釉蛋白基因cDNA全序列的克隆[J]. 中华口腔医学杂志, 2002, 37(4): 278-280 |
| 作者姓名： | 顾淑萍 史俊南 刘晗 郝建军 汪平 费俭 |
| 作者单位： | 1. 710032,西安,第四军医大学口腔医学院口腔内科 2. 上海中国科学院细胞生物学研究所基因工程组 |
| 基金项目： | 国家自然科学基金资助项目 (3 980 0 15 5，3 9970 792 ) |
| 摘 要： | 目的 筛选与小鼠牙胚发育相关的特异基因。方法 利用随机引物、反转录酶合成特异和非特异探针，采用差异显示方法筛选小鼠牙胚cDNA文库，挑选阳性克隆并测序。结果 得到6个阳性克隆，经测序证实其中1个克隆序列与大鼠成釉蛋白序列高度同源：其中用pTriplEX 3′引物测得的526个碱基序列与大鼠成釉蛋白基因5′端的32－580序列有497个碱基一致，5′引物测得的567个碱基序列与大鼠成釉蛋白基因3′端的1285－1854序列有533个碱基一致。结论 筛选到小鼠釉基质特异蛋白－成釉蛋白基因的cDNA全序列。 |
| 关 键 词： | 小鼠 成釉蛋白基因 cDNA 全序列 克隆 |
| 修稿时间： | 2001-01-03 |
Cloning of full length cDNA sequence of the mouse ameloblastin |
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| Shuping Gu,Junnan Shi,Han Liu,Jianjun Hao,Ping Wang,Jian Fei. Cloning of full length cDNA sequence of the mouse ameloblastin[J]. Chinese journal of stomatology, 2002, 37(4): 278-280 | |
| Authors: | Shuping Gu Junnan Shi Han Liu Jianjun Hao Ping Wang Jian Fei |
| Affiliation: | Department of Oral Medicine, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China. |
| Abstract: | OBJECTIVE: Screening for special genes of matrix proteins of dentin and enamel of mouse dental germ. METHODS: A cDNA library of dental germ of mouse was screened by differential display. The interesting clones were sequenced. RESULTS: Six positive clones were isolated from the cDNA library. The sequence of one of the six positive clones was homologous with the ameloblastin sequence of rat. There are 497 homologous base pairs between the 526 base pairs sequenced by pTriplEX 3' primer of this clone and the 32-580 sequence of the rat ameloblastin gene; and there are 533 homologous base pairs between the 567 base pairs sequenced by pTriplEX 5' primer of this clone and the 1285-1854 sequence of the rat ameloblastin gene. CONCLUSIONS: The full length cDNA sequence of the mouse ameloblastin was cloned. |
| Keywords: | Mice Dental germ DNA complementary |
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