耐甲氧西林金黄色葡萄球菌检测方法的比较

目的 对耐甲氧西林金黄色葡萄球菌(MRSA)的检测方法进行比较.方法 分别用mecA基因PCR扩增法、PBP2a胶乳凝集试验法、头孢西丁纸片扩散法和苯唑西林纸片扩散法对临床分离的135株金黄色葡萄球菌进行榆测.结果 mecA基因PCR扩增法显示阳性79株,阴性56株;PBP2a胶乳凝集试验法的敏感性和特异性分别为96.2%和100.0%;头孢西丁纸片扩散法的敏感性和特异性分别为94.9%和96.4%;苯唑西林纸片扩散法的敏感性和特异性分别为94.9%和62.5%.结论 PBP2a胶乳凝集试验法和头抱西丁纸片扩散法与mecA基因PCR扩增法的检测结果高度一致;苯唑西林纸片扩散法特异性低,不再适用于MRSA的检测.

A comparison of the methods for detection of methicillin resistant staphylococcus aureus

Objective To compare the methods for detecting methicillin resistant staphylococcus aureus(MRSA).Methods One hundred and thirty-five clinical isolates of staphylococcus aureus were examined with PCR amplification of the mecA gene,PBP2a test,disk diffusions method with cefoxitin and oxacillin,respectively.Results PCR amplification test showed 79 strains ositive and 56 strains nagetive.The sensitivity and specificity of PBP2a test were 96.2% and 100%,which were 94.9% and 96.4% by disk diffusion method with cefoxitin,94.9% and 62.5% by disk diffusion method with oxacillin,respectively.Conclusion The Results of PBP2a test and disk diffusions method with cefoxitin were highly accordant with those of PCR amplification method.The specificity of disk diffusions method with oxacillin is low and this method should not be used in the future.