| 沙利度胺的抗肝癌细胞株SMMC7721作用 |
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| 引用本文： | 鲁光平,潘骥群,于志坚,周雪峰,丁润生.沙利度胺的抗肝癌细胞株SMMC7721作用[J].江苏医药,2010,36(4). |
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| 作者姓名： | 鲁光平 潘骥群 于志坚 周雪峰 丁润生 |
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| 作者单位： | 1. 南通大学附属东台医院肿瘤科,东台,224200
2. 南通大学附属医院 |
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| 摘 要： | 目的 通过体外细胞培养研究沙利度胺(Tha)作用于肝细胞癌(HCC)细胞株
SMMC7721,探讨Tha抗HCC的作用机制.方法根据参考文献~(1]) 确定各组药物的最适作用浓度后,通过不同浓度Tha分别与HCC细胞SMMC7721体外培养,以MTT法检测细胞增殖活性,免疫组化法测血管内皮生长因子(VEGF)蛋白表达变化,流式细胞仪检测细胞凋亡率.结果不同浓度、不同时间Tha组细胞抑制率比较,差异有统计学意义(P<0.01).不同浓度Tha组作用于SMMC7721细胞48 h后,VEGF蛋白表达和细胞凋亡率随浓度增高而增加(P<0.05).结论Tha是有效的辅助抗肝癌药物.
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| 关 键 词： | 沙利度胺 SMMC7721 血管内皮生长因子 |
A study on the mechanism of thalidomide against HCC cell line SMMC7721 |
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| Abstract: | Objective To study the mechanism of thalidomide against hepatocellular carcinoma (HCC) cell line SMMC7721 in vitro.Methods According to the references, different concentrations of thalidomide were added into the SMMC7721 cell culture medium, respectively.The proliferative effect was assayed by MTT, the vascular endothelial growth factor (VEGF) was detected by immunohistochemical method,the apoptosis of the cell line was examined by flow cytometry.Results The depression rate was different among different concentrations and times of thalidomide (P< 0.01)).VEGF expression and cell apoptosis rate were increased as the thalidomide concentration elevated in 48 h (P<0.05).Conclusion Thalidomide is an effective adjuvant against HCC. |
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| Keywords: | Thalidomide SMMC7721 Vascular endothelial growth factor |
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