| 甲氧滴滴涕对雄性大鼠生精细胞的细胞周期影响 |
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| 引用本文： | 唐国华,贺修胜,何淑雅,唐新民. 甲氧滴滴涕对雄性大鼠生精细胞的细胞周期影响[J]. 生殖医学杂志, 2006, 15(2): 110-113 |
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| 作者姓名： | 唐国华 贺修胜 何淑雅 唐新民 |
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| 作者单位： | 湖南省南华大学肿瘤研究所,衡阳市,421001 |
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| 摘 要： | 目的探讨甲氧滴滴涕(MXC)对雄性大鼠生精细胞的细胞周期影响。方法实验动物分为对照组,溶剂组,5 d、10 d和15 d MXC实验组。用流式细胞仪检测染毒雄性大鼠生精细胞周期变化。结果随着MXC染毒时间延长与对照组比较,实验组G0/G1期与S期细胞数减少,G2/M期细胞数增多(P<0.05),单倍体细胞减少,二倍体与四倍体细胞增多(P<0.05);除5 d实验组二倍体与四倍体细胞荧光强度强外,其余各实验组的单倍体、二倍体和四倍体细胞荧光强度均减弱(P<0.05);溶剂组与对照组间的变化无显著性差异(P>0.05)。结论MXC可引起大鼠睾丸生精细胞S期抑制及G2期阻滞。
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| 关 键 词： | 甲氧滴滴涕 睾丸 生精细胞 细胞周期 |
| 文章编号： | 1004-3845(2006)02-0110-04 |
| 收稿时间： | 2004-09-30 |
| 修稿时间： | 2005-03-17 |
Effect of methoxychlor on germ cell cycle in male rat |
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| TANG Guo-hua,HE Xiu-sheng,HE Shu-ya,TANG Xin-min. Effect of methoxychlor on germ cell cycle in male rat[J]. Journal of Reproductive Medicine, 2006, 15(2): 110-113 |
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| Authors: | TANG Guo-hua HE Xiu-sheng HE Shu-ya TANG Xin-min |
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| Affiliation: | The Cancer Research Institute of Nan Hua University, Hengyang City 421001 |
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| Abstract: | Objective: To investigate the effect of methoxychlor(MXC) on cell cycle of germ cell in male rat.Methods: Male rats were exposed to MXC for different days(5,10 and 15 days) and the cyclic(changes) of germ cells were examined with flow cytometry.Results: The cell number of G_0/G_1 and S phase decreased,but that of G_2/M phase increased in experimental group when compared with control group(P<0.05).More haploid cells and fewer diploid and tetraploid cells were observed in the MXC exposed rats but not in those of day 5 group(P<0.05).The fluorescence intensity of haploid,diploid and tetraploid cells decreased in day 10 and day 15 group,but that of diploid and tetraploid cells increased in day 5 group.Conclusions: MXC can inhibit S phase of germ cell and arrest the cells in G_2 phase in rat testis. |
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| Keywords: | Methoxychlor Testis Germ cell Cell cycle |
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