即时荧光定量PCR微卫星分析技术检测少突胶质细胞肿瘤染色体1p/19q杂合性缺失

目的研究少突胶质细胞肿瘤染色体1p／19q杂合性缺失（LOH）情况。方法采用即时荧光定量PCR微卫星分析技术对28例少突胶质细胞肿瘤进行染色体1p／19qLOH检测。结果28例少突胶质细胞肿瘤染色体中有24例（85．7％）发生1pLOH;有18例（64．3％）发生19qLOH;有17例（60．7％）发生1p／19q联合性LOH;25例有1P或19qLOH。结论即时荧光定量PCR微卫星分析技术特异性高、方便快捷,可以用于石蜡包埋组织标本染色体杂合性缺失的检测。大多数少突胶质细胞肿瘤发生了1p／19qLOH。

Study of loss of heterozygosity in oligodendroglial tumors by real-time quantitative polymerase chain reaction-based microsatellite analysis

Objective To study the loss of heterozygosity (LOH) at chromosomes 1p or 19q in oligodendroglial tumors. Methods Twenty-eight cases of oligodendroglial tumors were enrolled into the study. Real-time quantitative polymerase chain reaction-based microsatellite analysis was performed on paraffin-embedded tumor tissues in order to study the status of chromosomes 1p and 19q. Results Among the 28 cases of oligodendroglial tumors, 24 cases (85.7%) showed 1p LOH, while 18 cases (64.3%) showed 19q LOH and 17 cases (60.7%) showed LOH of both 1p and 19q. LOH at 1p or 19q was present in 25 (89.3%) of the 28 cases. Conclusions Real-time quantitative polymerase chain reaction-based microsatellite analysis is a rapid and specific way in detecting LOH in paraffin-embedded tumor tissues. LOH at 1p or 19q is present in majority of the oligodendroglial tumors studied.