不同基原秦皮、香豆素单体抗菌作用对比研究

目的研究4种基原秦皮提取物、5种香豆素单体、5种单体混合物、苦枥白蜡树树皮已知香豆素指纹区样品、未知成分指纹区样品的体外抑菌作用。方法采用体外抑菌试验微量定量检测方法，测定最小抑菌浓度及最低杀菌浓度。结果4种基原秦皮苦枥白腊树、尖叶白腊树、白腊树、宿柱白腊树树皮均有明显抑菌、杀菌作用，抑菌强度依次为：宿柱白腊树〉苦枥白腊树〉尖叶白腊树〉白腊树。5种香豆素单体秦皮素、秦皮乙素对9种细菌均有明显抑菌、杀菌作用，秦皮甲素、秦皮苷对金黄色葡萄球菌、表皮葡萄球菌有一定抑菌、杀菌作用，抑菌作用依次为：秦皮素〉秦皮乙素〉秦皮甲素〉秦皮苷〉6，7-二甲氧基-8-羟基香豆素。苦枥白腊树树皮提取物对所试9种细菌均有明显抑菌作用；单体混合物，已知香豆素指纹区样品对金黄色葡萄球菌、表皮葡萄球菌、乙型溶血性链球菌、绿脓杆菌、大肠杆菌、肺炎克雷伯氏菌、卡他菌有明显抑菌作用；未知成分指纹区样品对金黄色葡萄球菌有明显抑菌作用，对表皮葡萄球菌、甲型溶血性链球菌、乙型溶血性链球菌、大肠杆菌、沙门氏菌也有一定抑菌作用。结论秦皮抑菌强弱与已知香豆素成分含量高低基本一致。秦皮中苷元的抑菌作用优于苷，5种香豆素类单体中秦皮素、秦皮乙素抑菌作用最强。

Comparative Study on Antibacterial Action of Coumarin Monomer and Ash Bark from Different Origins

Objective To study bacteriostasis in vitro of 4 kinds of origins ash bark extract, 5 kinds of coumarin monomer, 5 kinds of monomer mixture, known coumarin fingerprint region sample and unknown component fingerprint region of Fraxinus rhynchophylla Hance. Methods Bacteriostasis experiment in vitro and microamount and quantitative detection methods were adopted to measure minimum inhibitory concentration （MIC） and minimum bactericidal concentration （MBC）. Result Four kinds of origins ash bark including Fraxinus rhynchophyUa Hance, Fraxinus chinensis Roxb. var. acuminata Lingelsh., Fraxinus chinensis, Fraxinus stylosa Lingelsh. had obvious bacteriostasis and bactericidal action, bacteriostasis intensity order was Fraxinus stylosa Lingelsh. 〉Fraxinus rhynchophyUa Hance〉Fraxinus chinensis Roxb. var. acuminata Lingelsh. 〉Fraxinus chinensis. Five kinds of coumarin monomer： fraxetin, aesculetin had obvious bacteriostasis and bactericidal action, aesculin and fraxin had certain bacteriostasis and bactericidal action to Staphylococcus aureus, Staphylococcus epidermidis. Bacteriostasis and bactericidal action order was fraxetin 〉 aesculetin 〉 aesculin 〉 fraxin 〉 6,7-dichomethoxyl-8-Hydroxycoumarin. Fraxinus rhynchophyUa Hance extract had obvious bacteriostasis to 9 kinds of bacterium~ monomer mixture and coumarin fingerprint region sample had obvious bacteriostasis to Staphylococcus aureus, Staphylococcus epidermidis, beta streptococcus, aeruginosus Bacillus, Bacillus coli, Klebsiella pneumoniae, catarrh. Unknown component fingerprint region sample had obvious bacteriostasis to Staphylococcus aureus and certain bacteriostasis to Staphylococcus epidermidis, alpha streptococcus, beta streptococcus, Bacillus coli, salmonella. Conclusion The changes of ash bark bacteriostasis were basically identical with known coumarin component content. Aglycone bacteriostasis of ash bark was superior to glycoside, fraxetin and aesculetin bacteriostasis were most powerful in 5 kinds of coumarin monomer.