|
|||||
|
|
| 慢性阻塞性肺病大鼠骨骼肌蛋白降解的变化 | |
| 引用本文： | 杨天芸,徐卫国,罗勇,韩锋锋,陈迎春,张悦,万丹晶. 慢性阻塞性肺病大鼠骨骼肌蛋白降解的变化[J]. 上海交通大学学报(医学版), 2008, 28(5): 525-528 |
| 作者姓名： | 杨天芸 徐卫国 罗勇 韩锋锋 陈迎春 张悦 万丹晶 |
| 作者单位： | 上海交通大学医学院新华医院呼吸内科 上海200092(杨天芸，徐卫国，罗勇，韩锋锋，陈迎春，张悦)，复旦大学药学院放射药学教研组 上海200032(万丹晶) |
| 摘 要： | 目的探讨慢性阻塞性肺病(COPD)大鼠骨骼肌蛋白降解代谢变化的规律。方法利用熏香烟法制作COPD大鼠模型(COPD组),并设置对照组,每组20只。取伸趾长肌充氧离体孵育,采用高效液相-荧光法测量骨骼肌3-甲基组氨酸(3-MH)和酪氨酸(Tyr)的含量。结果孵育后,COPD组大鼠Tyr及3-MH的降解率与对照组比较,差异均有统计学意义(P<0.01,P<0.001)。结论COPD大鼠不仅全身骨骼肌总蛋白降解增加,作为机体蛋白质储存库重要组成部分的骨骼肌肌蛋白消耗更为明显。 |
| 关 键 词： | 慢性阻塞性肺病 骨骼肌 代谢 大鼠 |
| 文章编号： | 0258-5898(2008)05-0525-03 |
| 修稿时间： | 2008-03-14 |
Changes of skeletal muscle proteolysis in rats with chronic obstructive pulmonary disease |
|
| YANG Tian-yun,XU Wei-guo,LUO Yong,HAN Feng-feng,CHEN Ying-chun,ZHANG Yue,WAN Dan-jing. Changes of skeletal muscle proteolysis in rats with chronic obstructive pulmonary disease[J]. Journal of Shanghai Jiaotong University:Medical Science, 2008, 28(5): 525-528 | |
| Authors: | YANG Tian-yun XU Wei-guo LUO Yong HAN Feng-feng CHEN Ying-chun ZHANG Yue WAN Dan-jing |
| Abstract: | Objective To study the changes and rules of skeletal muscle proteolysis in rats with chronic obstructive pulmonary disease(COPD). Methods Rat COPD model(COPD group,n=20) was established by passive cigarette smoking,and the control group(n=20) was also established.After incubation in vivo with full oxygenation,contents of 3-methylhistidine(3-MH)and tyrosine(Tyr)in the extensor digitorium longus muscles were determined by high-performance liquid chromatography. Results After incubation,there were significant differences in the proteolytic rate of Tyr and 3-MH between COPD group and control group(P<0.01,P<0.001). Conclusion Total proteolytic rate and myofibrillar proteolytic rate in skeletal muscle are increased in rats with COPD. |
| Keywords: | chronic obstructive pulmonary disease skeletal muscle metabolism rat |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |