侧柏叶不同提取部位抑制胰脂肪酶及抗氧化活性筛选

目的:考察侧柏叶不同提取部位体外抑制胰脂肪酶活性和抗氧化活性。方法:对侧柏叶的醇提液、石油醚部位、乙酸乙酯部位、正丁醇部位、水部位采用体外抑制胰脂肪酶活性研究,并通过其清除1,1二苯基苦基苯肼(DPPH)自由基和Fe3+还原能力(FRAP)测定侧柏叶提取物的体外抗氧化作用。结果:侧柏叶提取物均有不同程度抑制胰脂肪酶活性,以乙酸乙酯部位效果最佳(IC50为26.09 mg·L-1),其次正丁醇部位(IC50为39.02 mg·L-1)和醇提物(IC50为98.20 mg·L-1),石油醚部位(IC50为188.27 mg·L-1)和水部位(IC50为325.28 mg·L-1)最弱。抗氧化活性依次为醇提液(DPPH IC50为7.59 mg·L-1,FRAP 4.40 mmol·g-1)乙酸乙酯部位(DPPH IC50为8.28 mg·L-1,FRAP 3.82 mmol·g-1)正丁醇部位(DPPH IC50为24.21mg·L-1,FRAP 1.77 mmol·g-1)水部位(DPPH IC50为27.53 mg·L-1,FRAP 1.38 mmol·g-1)石油醚部位。结论:初步确定侧柏叶抑制胰脂肪酶活性有效成分主要在乙酸乙酯与正丁醇部位,抗氧化及其相关成分主要在乙酸乙酯部位。

Study on Inhibition of Pancreatic Lipase and Antioxidation from Platycladus orientalis Leaves in vitro

Objective: To investigate the inhibition of pancreatic lipase and antioxidation of different extracts from Platycladus orientalis leaves in vitro. Method: The inhibitory activities of pancreatic lipase of ethanol extract, petroleum aether extract, ethyl acetate extract, n-butyl alcohol extract and water extract were assayed in vitro. The antioxidant activities of these extracts were assessed by two complementary test systems, namely 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and ferric reducing antioxidant power(FRAP) assay. Result: The ethyl acetate extract showed strong activity in the inhibitory activities of pancreatic lipase (IC₅₀ 26.09 mg · L^-1). The n-butanol extract (IC₅₀ 39.02 mg · L^-1) and ethanol extract (IC₅₀ 98.20 mg · L^-1) displayed lower activity. Petroleum ether extract (C₅₀ 188.27 mg · L^-1) and water fraction (IC₅₀ 325.28 mg · L^-1) have a little activity. The antioxidant activities:ethanol extract (DPPH IC₅₀ 7.59 mg · L^-1, FRAP 4.40 mmol ·g^-1) >ethyl acetate extract (DPPH IC₅₀ 8.28 mg · L^-1, FRAP 3.82 mmol · g^-1)>n-butanol extract (DPPH IC₅₀ 24.21 mg · L^-1, FRAP 1.77 mmol · g^-1)>water extract (DPPH IC₅₀ 27.53 mg · L^-1, FRAP 1.38 mmol · g^-1)>petroleum ether extract. Conclusion: The ethyl acetate extract and n-butanol extract were the components of inhibition pancreatic lipase. The ethyl acetate extract was the antioxidative components.