Determination of serum apolipoprotein B by competitive enzyme-linked immunoassay

Summary An enzyme-linked immunoassay was used to determine apolipoprotein B (apoB) in serum samples from 104 healthy adults aged 18–26 years. The method was compared to radial immunodiffusion (RID). Mean apoB values were 103±30 mg/dl and 105±31 mg/dl respectively. The correlation coefficient for apoB between the two methods wasr=0.70,p<0.001; the correlation coefficient between LDL-cholesterol and apoB measured by the ELISA wasr=0.73,p<0.001.This ELISA technique seems to be an appropriate method for measuring apoB in plasma, in the clinical laboratory and for detecting individuals with hyperapobetalipoproteinaemia.