Age-related oxidative decline of mitochondrial functions in rat brain is prevented by long term oral antioxidant supplementation |
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Authors: | Maria Bindu Bagh Ishita Guha Thakurta Mallika Biswas Prajna Behera Sasanka Chakrabarti |
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Institution: | (1) Department of Biochemistry, Institute of Postgraduate Medical Education and Research, 244B, Acharya J.C. Bose Road, Kolkata, 700020, India |
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Abstract: | A combination of antioxidants (N-acetyl cysteine, α-lipoic acid, and α-tocopherol) was selected for long term oral supplementation
study in rats for protective effects on age-related mitochondrial alterations in the brain. Four groups of rats were chosen:
young control (6–7 months); aged rats (22–24 months); aged rats (22–24 months) on daily antioxidant supplementation from 18 month
onwards and young rats (6–7 months) on daily antioxidant supplementation from 2 month onwards. The brain mitochondrial functional
parameters, status of antioxidant enzymes and accumulation of oxidative damage markers were measured in the four groups of
rats. A significant decrease in complex IV activity and a loss of transmembrane potential and phosphorylation capacity along
with an increased accumulation of oxidative damage markers and compromised antioxidant enzyme status were noticed in aged
rat brain mitochondria as compared to that in young controls, but in aged rats supplemented with oral antioxidants the mitochondrial
alterations were largely prevented. Antioxidant supplementation in young rats had no effect on mitochondrial parameters investigated
in this study. The results have implications in biochemical and functional deficits of brain during aging as well as in neurodegenerative
disorders. |
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