| 瘦素在胰岛素抵抗大鼠脂肪组织中的表达及意义 |
| |
| 引用本文： | 赵玉红,吴丽霞,关心,王伟,赵玉岩. 瘦素在胰岛素抵抗大鼠脂肪组织中的表达及意义[J]. 山东医药, 2011, 51(4): 11-12,15 |
| |
| 作者姓名： | 赵玉红 吴丽霞 关心 王伟 赵玉岩 |
| |
| 作者单位： | 1. 沈阳医学院沈洲医院,沈阳,110002
2. 中国医科大学附属第一医院 |
| |
| 基金项目： | 国家自然科学基金资助项目(30872724); 辽宁省自然科学基金项目(20082116); 辽宁省科技攻关层次计划项目(2008225009,2009225010-3) |
| |
| 摘 要： | 目的研究高脂肪膳食诱导的胰岛素抵抗（IR）大鼠脂肪组织中瘦素的变化规律及意义。方法 IR大鼠模型采用高脂肪膳食诱导并经钳夹技术证实。采用微型血糖仪、化学发光免疫分析法和生化比色法测定全血葡萄糖、血清胰岛素和血清游离脂肪酸（FFA）的变化。应用实时定量PCR和Western印迹杂交分析检测脂肪组织中瘦素的mRNA和蛋白质在IR状态下的表达情况。结果高脂肪膳食喂养4周后,实验组大鼠葡萄糖输注率较对照组降低,FFA、体质量、全血葡萄糖、血清胰岛素水平均高于对照组;高脂肪膳食诱导的IR大鼠脂肪组织中瘦素mRNA和蛋白质的表达均较对照组增加（P〈0.05）。结论高脂肪膳食可诱导大鼠产生IR,并增强脂肪组织中瘦素基因的转录和翻译。瘦素可能是参与调节脂肪—胰岛轴的主要因素之一。
|
| 关 键 词： | 瘦素 脂肪 胰岛素抵抗 基因表达 |
Expression of leptin in rat fat tissue with insulin resistance |
| |
| ZHAO Yu-hong,WU Li-xia,GUAN Xin,WANG Wei,ZHAO Yu-yan. Expression of leptin in rat fat tissue with insulin resistance[J]. Shandong Medical Journal, 2011, 51(4): 11-12,15 |
| |
| Authors: | ZHAO Yu-hong WU Li-xia GUAN Xin WANG Wei ZHAO Yu-yan |
| |
| Affiliation: | ZHAO Yu-hong1,WU Li-xia,GUAN Xin,WANG Wei,ZHAO Yu-yan(1 The Second Affiliated Hospital of Shenyang Medical College,Shenyang 110002,P.R.China) |
| |
| Abstract: | Objective To evaluate the expression of leptin in rat fat tissue with insulin resistance(IR) induced by high-fat diet.Methods Rat model with IR induced by high-fat diet was confirmed by euglycemic clamp.Insulin and free fatty acid(FFA) were measured by chemiluminescent immunoassay and biochemistic chromatometry method.Expression of leptin in fat tissue was identified by real-time PCR and Western blot analysis,respectively.Results After 4 weeks high-fat diet,the glucose infusion rate of IR rats was higher th... |
| |
| Keywords: | leptin fat insulin resistance gene expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
