TLR7基因单核苷酸多态性与食管癌易感性的相关性研究

目的本研究旨在探讨toll样受体7基因(TLR7)3'端非编码序列microRNA-1265(miR-1265)结合位点单核苷酸多态性rs72552316与食管癌易感性的关系。方法采用单中心为基础的病例对照研究。在这项研究中,聚合酶链反应(PCR)和直接测序被用于204食管癌病例和200例健康对照TLR7基因分型。rs72552316影响miR-1265对TLR7蛋白表达的调控由免疫组化法检测。结果与携带野生型纯合子AA基因型比较,携带rs72552316的GG基因型个体患食管癌的风险增加(OR=2.180,95%CI=1.152-4.127,P=0.019)。此外,在食管癌组织中检测TLR7的表达,这表明TLR7蛋白水平可能与SNP rs72552316相关。结论 Toll样受体7基因3?端非编码序列miR-1265结合位点单核苷酸多态性rs72552316与食管癌易感性相关。miR-1265/TLR7轴可能在食管癌的发生发展中起重要作用。

Correlation between single nucleotide polymorphisms of TLR7 gene and susceptibility to esophageal cancer

Objective To investigate the relationship of a functional variant (rs72552316)at microRNA-1265 (miR-1265) binding site in toll-like receptor 7 gene and the risk of esophageal cancer.Methods A single center-based case-control study was conducted.In this study,the polymerase chain reaction (PCR) and direct sequencing were used for genotype sequence variants of TLR7 in 204 esophageal cancer cases and 200 controls.The modification of rs72552316 on the binding of miR-1265 to TLR7 protein was measured by Immunohistochemistry assay.Results Individuals carrying the GG genotypes for the rs72552316 were associated significantly with increased risk of esophageal cancer comparing with those carrying wild-type homozygous CC genotypes (OR =2.180,95％CI=1.152-4.127,P =0.019).Furthermore,the expression of TLR7 was detected in the esophageal cancer tissue,suggesting that protein levels of TLR7 might be associated with SNP rs72552316.Conclusion Risk of esophageal cancer was associated with a functional variant at miR1265 binding site in toll-like receptor 7 gene.miR-1265/TLR7 axis may play an important role in the development of esophageal cancer.