Determination of MK-0476 in human plasma by liquid chromatography |
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| Affiliation: | 1. Department of Building Science, School of Architecture, Tsinghua University, Beijing 100084, China;2. Beijing Key Laboratory of Indoor Air Quality Evaluation and Control, Beijing 100084, China;1. Department of Mathematics, National University of Singapore, Kent Ridge, Singapore 119260, Republic of Singapore;2. Division of Mathematical Sciences, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371, Republic of Singapore;3. Zhejiang Lab, Hangzhou 311100, China;1. Medicinal Chemistry Research Laboratories, Ono Pharmaceutical Co., Ltd, 3-1-1 Sakurai, Shimamoto-cho, Mishima-gun, Osaka 618-8585, Japan;2. Exploratory Pharmaceutics Group Pharmaceutical Technology Laboratories, Ono Pharmaceutical Co., Ltd, 3-1-1 Sakurai, Shimamoto-cho, Mishima-gun, Osaka 618-8585, Japan;3. Department of Biology & Pharmacology, Ono Pharmaceutical Co., Ltd, 3-1-1 Sakurai, Shimamoto-cho, Mishima-gun, Osaka 618-8585, Japan;1. New Drug Screening Center, Jiangsu Center for Pharmacodynamics Research and Evaluation, China Pharmaceutical University, China;2. Shuangyun BioMed Sci & Tech (Suzhou) Co., Ltd, China;1. Bezmialem Vakif University, Faculty of Pharmacy, Department of Analytical Chemistry, Fatih, 34093 Istanbul, Turkey;2. Department of Pharmacy, University of Chieti–Pescara “G. d’Annunzio”, Via dei Vestini 31, Chieti 66100, Italy;3. Department of Chemistry and Biochemistry, Florida International University, 11200 SW 8th St, Miami, FL 33199, USA |
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| Abstract: | ![]()
A simple and accurate assay for quantitating MK-0476 [sodium 1-(((1(R)-(3-(2-(7-chloro-2-quinolinyl)-(E)-ethenyl)phenyl)(3-(2-(1-hydroxy-1-methylethyl)phenyl)propyl)thio)-methyl)cyclopropane)acetate], which is a potent and selective leukotriene D4-receptor antagonist, in human plasma has been developed. The method involves precipitation of protein and reversed-phase liquid chromatography with fluorescence detection. The assay is linear in the range of 30–3000 ng ml−1 of MK-0476, and the limit of detection is 5 ng ml−1. The interday precision (% relative standard deviation) values of this method at 51 and 2040 ng ml−1 are 10 and 3%, respectively. The interday accuracy values at these concentrations are 94 and 104%, respectively. The absolute recovery of MK-0476 is 99%. The utility of this method to determine plasma concentrations of MK-0476 in humans receiving the drug orally was demonstrated. |
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